Analysis of pulmonary surfactant in rat lungs after inhalation of nanomaterials: Fullerenes, nickel oxide and multi-walled carbon nanotubes
Autor: | Isamu Tanaka, Toshihiko Myojo, Byeong-Woo Lee, Akira Ogami, Motoi Todoroki, Makoto Yamamoto, Yasuo Morimoto, Takako Oyabu, Kenichiro Nishi, Chikara Kadoya |
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Rok vydání: | 2015 |
Předmět: |
Male
Materials science Biomedical Engineering Inhaled air Toxicology Nanomaterials Pulmonary surfactant Nickel medicine Animals Surface Tension Organic chemistry Rats Wistar Lung Phospholipids Aerosols Inhalation exposure Inhalation Exposure medicine.diagnostic_test Inhalation Nanotubes Carbon Proteins Surfactant protein D Pulmonary Surfactants respiratory system Pulmonary Surfactant-Associated Protein D Rats respiratory tract diseases Bronchoalveolar lavage medicine.anatomical_structure Nanoparticles Fullerenes Bronchoalveolar Lavage Fluid Nuclear chemistry |
Zdroj: | Nanotoxicology. :1-10 |
ISSN: | 1743-5404 1743-5390 |
DOI: | 10.3109/17435390.2015.1039093 |
Popis: | The health risks of inhalation exposure to engineered nanomaterials in the workplace are a major concern in recent years, and hazard assessments of these materials are being conducted. The pulmonary surfactant of lung alveoli is the first biological entity to have contact with airborne nanomaterials in inhaled air. In this study, we retrospectively evaluated the pulmonary surfactant components of rat lungs after a 4-week inhalation exposure to three different nanomaterials: fullerenes, nickel oxide (NiO) nanoparticles and multi-walled carbon nanotubes (MWCNT), with similar levels of average aerosol concentration (0.13-0.37 mg/m(3)). Bronchoalveolar lavage fluid (BALF) of the rat lungs stored after previous inhalation studies was analyzed, focusing on total protein and the surfactant components, such as phospholipids and surfactant-specific SP-D (surfactant protein D) and the BALF surface tension, which is affected by SP-B and SP-C. Compared with a control group, significant changes in the BALF surface tension and the concentrations of phospholipids, total protein and SP-D were observed in rats exposed to NiO nanoparticles, but not in those exposed to fullerenes. Surface tension and the levels of surfactant phospholipids and proteins were also significantly different in rats exposed to MWCNTs. The concentrations of phospholipids, total protein and SP-D and BALF surface tension were correlated significantly with the polymorphonuclear neutrophil counts in the BALF. These results suggest that pulmonary surfactant components can be used as measures of lung inflammation. |
Databáze: | OpenAIRE |
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