No Evidence of Helicobacter pylori Sequences in Pancreatic Juices of Patients Affected by Chronic Pancreatitis
| Autor: | A. Armuzzi, Antonio Gasbarrini, C. Di Campli, N Gentiloni, R Nocente, Roberto Burioni, Jian Wu, Guido Costamagna, Mark A. Zern, G. Gasbarrini |
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| Přispěvatelé: | Di Campli, C, Nocente, R, Costamagna, G, Gentiloni, N, Burioni, Roberto, Wu, J, Armuzzi, A, Zern, Ma, Gasbarrini, G, Gasbarrini, A. |
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Pancreatic disease Spirillaceae medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Gastroenterology Endocrinology Pancreatic Juice Internal medicine Humans Medicine Endoscopic retrograde cholangiopancreatography Helicobacter pylori biology medicine.diagnostic_test business.industry biology.organism_classification medicine.disease Urease medicine.anatomical_structure Pancreatitis Oncology Superinfection Chronic Disease Pancreatic juice business Pancreas |
| Zdroj: | International Journal of Gastrointestinal Cancer. 28:181-186 |
| ISSN: | 1537-3649 |
| DOI: | 10.1385/ijgc:28:3:181 |
| Popis: | Background: The course of chronic pancreatitis is often unpredictable and many factors are likely to be involved in the progression of the disease. In physiological condition, pancreatic juice exerts significant antibacterial activity, which is impaired in patients with chronic pancreatitis. Aim: Hypothesizing that Helicobacter pylori could, in these conditions, lead to an ascending infection, we aimed to assess the presence of H. pylori sequences in pancreatic juices of patients with chronic pancreatitis. Methods: 40 patients (mean age 52±3 yr) with alcoholic chronic pancreatitis and H. pylori infection were examined. Pancreatic juices were collected during endoscopic retrograde cholangiopancreatography. Using polymerase chain reaction (PCR) with two primers homologous to a portion of urease-C gene, H. pylori DNA was detected. Gastric biopsies, microscopically positive to H. pylori were used as positive controls. Results: All gastric biopsies produced H. pylori-specific DNA products. Conversely, no H. pylori urease-C gene sequences have been detected in any of the pancreatic juices. Conclusion: Our data suggest that the impaired antibacterial activity of pancreatic juices in patients affected by chronic pancreatitis does not have a permissive role for a superimposingH. pyloriinfection in the pancreas. The possibility that Helicobacter species other than pylori may be involved in a superimposing infection requires further investigation. |
| Databáze: | OpenAIRE |
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