The structured organization of Deinococcus radiodurans ’ cell envelope

Autor:	Farci, Domenica, Haniewicz, Patrycja, Piano, Dario
Rok vydání:	2022
Předmět:	Fysikalisk kemi Multidisciplinary SDBC cryo-electron crystallography Cryoelectron Microscopy Cell Membrane Type IV piliation system cryo-electron tomography Physical Chemistry Microbiology S-layer Mikrobiologi Bacterial Proteins Cell Wall Deinococcus
Zdroj:	Proc Natl Acad Sci U S A.
ISSN:	1091-6490 0027-8424
Popis:	Surface layers (S-layers) are highly ordered coats of proteins localized on the cell surface of many bacterial species. In these structures, one or more proteins form elementary units that self-assemble into a crystalline monolayer tiling the entire cell surface. Here, the cell envelope of the radiation-resistant bacterium Deinococcus radiodurans was studied by cryo-electron microscopy, finding the crystalline regularity of the S-layer extended into the layers below (outer membrane, periplasm, and inner membrane). The cell envelope appears to be highly packed and resulting from a three-dimensional crystalline distribution of protein complexes organized in close continuity yet allowing a certain degree of free space. The presented results suggest how S-layers, at least in some species, are mesoscale assemblies behaving as structural and functional scaffolds essential for the entire cell envelope.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e52eb426376e1ac53bf8d07f96e3da1 https://doi.org/10.1073/pnas.2209111119 Zobrazit plný text záznamu