Oxidative stress and antioxidant status in beta-thalassemia heterozygotes
| Autor: | Marina Ibelli Pereira Rocha, Isabeth da Fonseca Estevão, Claudia Regina Bonini-Domingos, Luciana de Souza Ondei, Sandro Percário, Marcela Augusta de Souza Pinhel, Dorotéia Rossi Silva Souza |
|---|---|
| Přispěvatelé: | Universidade Estadual de Goiás (UEG), Universidade Estadual Paulista (Unesp), Universidade Federal do Para, Fundacao Faculdade Regional de Medicina de Sao Jose do Rio Preto |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Antioxidant Thiobarbituric acid medicine.medical_treatment Lipid peroxidation Trolox equivalent antioxidant capacity Antioxidantes Talassemia beta Peróxidos lipídicos medicine.disease_cause Beta-thalassemia chemistry.chemical_compound Estresse oxidativo Internal medicine medicine Beta-globins Mutação medicine.diagnostic_test biology lcsh:RC633-647.5 Chemistry Substância reactiva ao ácido tiobarbitúrico lcsh:Diseases of the blood and blood-forming organs Hematology Original Articles Ferritin Beta globina Endocrinology Biochemistry Oxidative stress Mutation Serum iron biology.protein Thiobarbituric acid reactive substances Hemoglobin |
| Zdroj: | Revista Brasileira de Hematologia e Hemoterapia Revista Brasileira de Hematologia e Hemoterapia v.35 n.6 2013 Revista brasileira de hematologia e hemoterapia Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC) instacron:ABHHTC Revista Brasileira de Hematologia e Hemoterapia, Vol 35, Iss 6, Pp 409-413 (2013) Revista Brasileira de Hematologia e Hemoterapia, Volume: 35, Issue: 6, Pages: 409-413, Published: 2013 SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da UFPA Universidade Federal do Pará (UFPA) instacron:UFPA |
| ISSN: | 1806-0870 1516-8484 |
| Popis: | Made available in DSpace on 2014-09-30T18:18:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2013Bitstream added on 2014-10-01T14:03:25Z : No. of bitstreams: 1 S1516-84842013000600409.pdf: 380660 bytes, checksum: 6414b1538be066596281f3f2828e5b54 (MD5) Background:Several studies have evaluated the oxidant and antioxidant status of thalassemia patients but most focused mainly on the severe and intermediate states of the disease. Moreover, the oxidative status has not been evaluated for the different beta-thalassemia mutations.Objective:To evaluate lipid peroxidation and Trolox equivalent antioxidant capacity in relation to serum iron and ferritin in beta thalassemia resulting from two different mutations (CD39 and IVS-I-110) compared to individuals without beta-thalassemia.Methods:One hundred and thirty subjects were studied, including 49 who were heterozygous for beta-thalassemia and 81 controls. Blood samples were subjected to screening tests for hemoglobin. Allele-specific polymerase chain reaction was used to confirm mutations for beta-thalassemia, an analysis of thiobarbituric acid reactive species was used to determine lipid peroxidation, and Trolox equivalent antioxidant capacity evaluations were performed. The heterozygous beta-thalassemia group was also evaluated for serum iron and ferritin status.Results:Thiobarbituric acid reactive species (486.24 ± 119.64 ng/mL) and Trolox equivalent antioxidant capacity values (2.23 ± 0.11 mM/L) were higher in beta-thalassemia heterozygotes compared to controls (260.86 ± 92.40 ng/mL and 2.12 ± 0.10 mM/L, respectively; p-value < 0.01). Increased thiobarbituric acid reactive species values were observed in subjects with the CD39 mutation compared with those with the IVS-I-110 mutation (529.94 ± 115.60 ng/mL and 453.39 ± 121.10 ng/mL, respectively; p-value = 0.04). However, average Trolox equivalent antioxidant capacity values were similar for both mutations (2.20 ± 0.08 mM/L and 2.23 ± 0.12 mM/L, respectively; p-value = 0.39). There was no influence of serum iron and ferritin levels on thiobarbituric acid reactive species and Trolox equivalent antioxidant capacity values.Conclusion:This study shows an increase of oxidative stress and antioxidant capacity in beta-thalassemia heterozygotes, mainly in carriers of the CD39 mutation. Universidade Estadual de Goias Universidade Estadual Paulista Universidade Federal do Para Fundacao Faculdade Regional de Medicina de Sao Jose do Rio Preto Universidade Estadual Paulista |
| Databáze: | OpenAIRE |
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