Microvesicles Correlated with Components of Metabolic Syndrome in Men with Type 2 Diabetes Mellitus and Lowered Testosterone Levels but Were Unaltered by Testosterone Therapy

Autor: Line Velling Magnussen, Tine Bo Nielsen, Marianne Andersen, Jaco Botha, Aase Handberg, Morten Hjuler Nielsen, Kurt Højlund
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Blood Glucose
Male
medicine.medical_specialty
Article Subject
Hormone Replacement Therapy
Endocrinology
Diabetes and Metabolism
CD36
030204 cardiovascular system & hematology
Biology
lcsh:Diseases of the endocrine glands. Clinical endocrinology
Circulating microvesicle
03 medical and health sciences
0302 clinical medicine
Endocrinology
Double-Blind Method
Cell-Derived Microparticles
Internal medicine
Diabetes mellitus
medicine
Journal Article
Humans
Insulin
Testosterone
Gamma-glutamyltransferase
Aged
Metabolic Syndrome
lcsh:RC648-665
Microvesicle
Middle Aged
medicine.disease
Flow Cytometry
Microvesicles
030104 developmental biology
Treatment Outcome
Diabetes Mellitus
Type 2
biology.protein
Clinical Study
Body Composition
Metabolic syndrome
Insulin Resistance
Waist Circumference
Zdroj: Botha, J, Velling Magnussen, L, Nielsen, M H, Nielsen, T B, Højlund, K, Andersen, M S & Handberg, A 2017, ' Microvesicles Correlated with Components of Metabolic Syndrome in Men with Type 2 Diabetes Mellitus and Lowered Testosterone Levels but Were Unaltered by Testosterone Therapy ', Journal of Diabetes Research, vol. 2017, 4257875 . https://doi.org/10.1155/2017/4257875
Botha, J, Velling Magnussen, L, Nielsen, M H, Nielsen, T B, Højlund, K, Andersen, M S & Handberg, A 2017, ' Microvesicles Correlated with Components of Metabolic Syndrome in Men with Type 2 Diabetes Mellitus and Lowered Testosterone Levels But Were Unaltered by Testosterone Therapy ', Journal of Diabetes Research, vol. 2017, 4257875 . https://doi.org/10.1155/2017/4257875
Journal of Diabetes Research, Vol 2017 (2017)
Journal of Diabetes Research
Popis: Aims. To investigate how circulating microvesicle phenotypes correlate with insulin sensitivity, body composition, plasma lipids, and hepatic fat accumulation. We hypothesized that changes elicited by testosterone replacement therapy are reflected in levels of microvesicles. Methods. Thirty-nine type 2 diabetic males with lowered testosterone levels were assigned to either testosterone replacement therapy or placebo and evaluated at baseline and after 24 weeks. Microvesicles were analysed by flow cytometry and defined as lactadherin-binding particles within the 0.1–1.0 μm gate. Microvesicles of platelet, monocyte, and endothelial cell origin were identified by cell-specific markers and their expression of CD36 was investigated. Results. Triglycerides correlated positively with all investigated microvesicle phenotypes in this study (p<0.05), and indicators of hepatic fat accumulation, alanine aminotransferase, and gamma glutamyltransferase correlated with platelet and endothelial microvesicles and CD36-expressing microvesicles from platelets and monocytes (p<0.05). BMI, waist circumference, and fat percentage correlated with CD36-expressing monocyte microvesicles (p<0.05), while insulin sensitivity did not correlate with any microvesicle phenotypes. Microvesicle levels were unaffected by testosterone therapy. Conclusions. Metabolic syndrome components and hepatic fat accumulation correlated with microvesicle phenotypes, supporting the involvement of especially CD36 on monocytes in metabolic syndrome pathogenesis. Although testosterone therapy improved body composition measures, microvesicle phenotype levels were unaffected. This trial was registered at ClinicalTrials.gov (NCT01560546).
Databáze: OpenAIRE
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