Protective role of oligomeric proanthocyanidin complex against hazardous nodularin-induced oxidative toxicity in Carassius auratus lymphocytes
| Autor: | Wendi Fang, Lu Liping, Xiuying Jia, Hangjun Zhang, Xiao Wenfeng |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Environmental Engineering Antioxidant Health Toxicology and Mutagenesis medicine.medical_treatment Oxidative phosphorylation Nod medicine.disease_cause Peptides Cyclic Antioxidants chemistry.chemical_compound Goldfish Malondialdehyde medicine Animals Environmental Chemistry Proanthocyanidins Lymphocytes Waste Management and Disposal Cells Cultured bcl-2-Associated X Protein chemistry.chemical_classification Glutathione Peroxidase Reactive oxygen species Caspase 3 Superoxide Dismutase Chemistry Glutathione Pollution Molecular biology Oxidative Stress Glutathione Reductase Proto-Oncogene Proteins c-bcl-2 Biochemistry Apoptosis Toxicity Female Reactive Oxygen Species Oxidative stress |
| Zdroj: | Journal of Hazardous Materials. 274:247-257 |
| ISSN: | 0304-3894 |
| DOI: | 10.1016/j.jhazmat.2014.04.020 |
| Popis: | Nodularin (NOD) is a hazardous material widely detected in water blooms. Fish immune cells are extremely vulnerable to NOD-induced oxidative stress. Oligomeric proanthocyanidin complex (OPC), extracted from grapeseed, was used as an antioxidant to eliminate reactive oxygen species and prevent apoptotic effects. Carassius auratus lymphocytes were treated with different concentrations (0, 10, 100, and 1,000 μg/L) of OPC and a constant dose (100 μg/L) of NOD for 12h in vitro. OPC inhibited mitosis by decreasing intracellular levels of oxidative stress, regulating antioxidant enzymes (CAT, SOD, GPx, GR, and GST), mediating bcl-2 family proteins, and deactivating caspase-3. Glutathione (GSH) levels in group V (NOD 100 μg/L; OPC 1,000 μg/L) showed a twofold increase compared with corresponding levels in group II (NOD 100 μg/L). Structure parameters of NOD and NOD-GSH were calculated using SYBYL 7.1 software. ClogP and HINK logP values of NOD-GSH decreased by 10.4- and 2.3-fold, respectively, compared with corresponding values of NOD. OPC-stimulated GSH can lower the lipophilicity and polarity of NOD. OPC, as a protective agent, can alleviate NOD-induced toxicity in C. auratus lymphocytes by regulating oxidative stress and inducing NOD-GSH detoxification. |
| Databáze: | OpenAIRE |
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