The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii
| Autor: | A.S. Chunaev, S. N. Korolkov, A.S. Basov, I.L. Dumler, M.N. Garnovskaya |
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| Rok vydání: | 1990 |
| Předmět: |
Rhodopsin
G-protein Light G protein Protein subunit Immunoblotting Biophysics Chlamydomonas reinhardtii Peptide GTPase Sulfur Radioisotopes Biochemistry Antibodies GTP Phosphohydrolases Structural Biology GTP-Binding Proteins Genetics Transducin Molecular Biology chemistry.chemical_classification biology Binding protein Chlamydomonas Cell Biology biology.organism_classification Molecular Weight chemistry Guanosine 5'-O-(3-Thiotriphosphate) GTPγ[35S] biology.protein Eyespot |
| Zdroj: | FEBS letters. 270(1-2) |
| ISSN: | 0014-5793 |
| Popis: | The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTP gamma[35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' alpha-subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate beta-subunit peptide, two additional proteins with apparent Mr 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of Ch. reinhardtii. |
| Databáze: | OpenAIRE |
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