SMC1 involvement in fragile site expression
| Autor: | P.A. Benedetti, Maria Luisa Focarelli, Lorenzo Brait, Thomas Ried, Paolo Vezzoni, Luciana Chessa, Alberto Albertini, Antonio Musio, Manuela Tilenni, Tullio Mariani, Esterina Indino, Cristina Montagna |
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| Rok vydání: | 2005 |
| Předmět: |
Aphidicolin
DNA Replication Cell cycle checkpoint Chromosomal Proteins Non-Histone Context (language use) Cell Cycle Proteins Ataxia Telangiectasia Mutated Proteins Biology Protein Serine-Threonine Kinases Chromosomes Histones chemistry.chemical_compound Genetics Humans Neoplastic transformation Enzyme Inhibitors Molecular Biology Genetics (clinical) Cell Nucleus Chromosomal fragile site Chromosome Fragile Sites Tumor Suppressor Proteins DNA replication General Medicine Fibroblasts Oligonucleotides Antisense DNA Replication Fork Molecular biology Cell biology DNA-Binding Proteins chemistry Chromosome Fragile Site RNA Interference HeLa Cells |
| Zdroj: | Human molecular genetics 14 (2005): 525–533. doi:10.1093/hmg/ddi049 info:cnr-pdr/source/autori:Musio A, Montagna C, Mariani T, Tilenni M, Focarelli ML, Brait L, Indino E, Benedetti PA, Chessa L, Albertini A, Ried T, Vezzoni P./titolo:SMC1 involvement in fragile site expression/doi:10.1093%2Fhmg%2Fddi049/rivista:Human molecular genetics (Print)/anno:2005/pagina_da:525/pagina_a:533/intervallo_pagine:525–533/volume:14 |
| ISSN: | 0964-6906 |
| DOI: | 10.1093/hmg/ddi049 |
| Popis: | Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto, which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (approximate to500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer. |
| Databáze: | OpenAIRE |
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