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Physical exercise is a planned regular and repeated activities in a certain intensity to improve health. Based on duration, there are two kinds of physical exercise. Physical exercise with shorter amount of time is called acute physical exercise. While physical exercise with longer amount of time is called chronic physical exercise. Physical exercise will make some changes in the body, such as blood oxygen levels. Some studies showed the influence of physical exercise on oxygen saturation but some studies had the opposite result. Therefore, this study was carried out to investigate the effect of acute physical exercise on oxygen saturation. This study was a field experimental with pre-post one group test design that was conducted to 32 Medical Faculty student basketball players of Sam Ratulangi University. Each subject had to play basketball for 20 minutes. Value of oxygen saturation was checked using pulse oximeter before exercise and immediately after exercise. Case analysis use paired samples T test. This study has found an increase on oxygen saturation after the acute physical exercise (p=0,041;p |