Degradation of a Plasticizer, di-n-Butylphthalate by Delftia sp. TBKNP-05
| Autor: | Neelakanteshwar K. Patil, Yogesh S. Shouche, Timmanagouda B. Karegoudar, Rajesh R. Kundapur |
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| Rok vydání: | 2006 |
| Předmět: |
Phthalic Acids
Applied Microbiology and Biotechnology Microbiology Mineralization (biology) Esterase chemistry.chemical_compound Plasticizers Hydroxybenzoates Delftia Phylogeny chemistry.chemical_classification Chromatography biology Phthalate Plasticizer Biological Transport General Medicine Biodegradation Ribosomal RNA biology.organism_classification Dibutyl Phthalate Oxygen Biodegradation Environmental Enzyme Biochemistry chemistry Bacteria circulatory and respiratory physiology |
| Zdroj: | Current Microbiology. 52:225-230 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-005-0258-9 |
| Popis: | A bacterial strain Delftia sp. TBKNP-05 isolated by para-hydroxybenzoate enrichment technique is capable of degrading di-n-butylphthalate (DBP) as a sole source of carbon and energy. Analysis of intermediates by thin layer chromatography and high performance liquid chromatography indicated the presence of monobutylphthalate (MBP), phthalate (PA), and protocatechuate (PCA). The washed cells grown on DBP and PA showed appreciable oxidation of DBP, MBP, PA, and PCA. The enzyme activities in cell free extracts of Delftia sp. TBKNP-05 exhibited the presence of DBP esterase, MBP esterase, PA-dioxygenase, and protocatechuate 4, 5-dioxygenase. The protocatechuate is metabolized by a meta-cleavage pathway leading to further mineralization of the compound in this bacterium. |
| Databáze: | OpenAIRE |
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