A new generation of Bt maize for control of fall armyworm ( Spodoptera frugiperda )
| Autor: | Hallison Vertuan, Graham P. Head, Patrick M Dourado, Renato J. Horikoshi, Heather M. Anderson, Jianguo Tan, Geraldo U. Berger, Samuel Martinelli, Cara Griffith, Adam Evans, Castro Ancideriton A, Kimberly Morrell, Peter Asiimwe, Marcia Ometto de Mello Alves José |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Integrated pest management Bt pyramids Spodoptera Biology Zea mays 01 natural sciences Insecticide Resistance Crop Hemolysin Proteins Bacterial Proteins Animals Humans Bioassay Research Articles MON 95379 Larva Genetically modified maize Bacillus thuringiensis Toxins fungi Infant Newborn Special Issue: Insect Control Agents ‐ Discovery and Neurotoxicology (ACS Fall 2019) Spodoptera frugiperda General Medicine Plants Genetically Modified biology.organism_classification Endotoxins 010602 entomology Agronomy Insect Science FAW Fall armyworm Agronomy and Crop Science Research Article 010606 plant biology & botany Field conditions |
| Zdroj: | Pest Management Science |
| ISSN: | 1526-4998 1526-498X |
| Popis: | BACKGROUND The pyramided genetically modified maize (Zea mays [L.]) event MON 95379, expressing the Cry1B.868 and Cry1Da_7 proteins, was designed to protect against larval feeding damage by the fall armyworm, Spodoptera frugiperda (FAW). Here, we conducted laboratory, greenhouse, and field studies to assess the dose and field efficacy of MON 95379 against FAW and inform the development of insect resistance management plans. RESULTS The Cry1B.868 and Cry1Da_7 proteins were active against susceptible FAW neonates in diet‐incorporation bioassays: median lethal concentration [LC50] (95% CI) = 62.8 (42.6–87.6) μg/ml diet for Cry1B.868 and 9.4 (5.3–18.6) μg/ml diet for Cry1Da_7. In laboratory leaf disc bioassays, MON 95379 maize and experimental maize lines expressing the individual components were effective in controlling susceptible FAW. In whole‐plant assays, MON 95379 controlled FAW resistant to the Cry1A.105 and Cry2Ab2 proteins. Likewise, under field conditions, MON 95379 maize expressing Cry1B.868 and Cry1Da_7 was highly effective at protecting plants against the larval feeding of FAW. CONCLUSIONS The expression of Cry1B.868 and Cry1Da_7 in MON 95379 consistently protected maize plants against larval feeding by FAW and represents an alternative to manage trait resistance issues in South America. © 2021 Bayer Crop Science‐US. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. The pyramided genetically modified maize (Zea mays [L.]) event MON 95379, expressing the Cry1B.868 and Cry1Da_7 proteins was designed to protect against larval feeding damage of the fall armyworm, Spodoptera frugiperda (FAW). The expression of Cry1B.868 and Cry1Da_7 in MON 95379 consistently protected maize plants against larval feeding of FAW and represents an alternative to manage trait resistance issues in South America. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text