Association of the interferon-gamma single nucleotide polymorphism +874(T/A) with response to immunosuppressive therapy in patients with severe aplastic anemia
| Autor: | Hong Chang, Jia-You Zhang, Wen-Tong Meng, Hongbing Ma, Fan Zeng, Xiao-Yan Mu, Ting Liu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Adolescent Genotype Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Interferon-gamma Young Adult Immune system Gene Frequency Blood plasma medicine Humans SNP Interferon gamma Aplastic anemia Molecular Biology Gene Aged Aged 80 and over Immunosuppression Therapy Anemia Aplastic Cell Biology Hematology Middle Aged medicine.disease Pharmacogenetics Case-Control Studies Immunology Molecular Medicine Female Immunosuppressive Agents medicine.drug |
| Zdroj: | Blood Cells, Molecules, and Diseases. 45:313-316 |
| ISSN: | 1079-9796 |
| DOI: | 10.1016/j.bcmd.2010.09.003 |
| Popis: | The aim of this study was to investigate whether a single nucleotide polymorphism (SNP) +874T/A of interferon-gamma (IFN-γ) correlates with response to immunosuppressive therapy. Amplification refractory mutation system-polymerase chain reaction was used to amplify the polymorphic segments of the IFN-γ +874T/A gene in the samples obtained from 54 patients with aplastic anemia and 51 healthy adults. Further, enzyme linked immunosorbent assay was used to assay IFN-γ levels in the blood plasma of 35 patients with severe aplastic anemia before immune suppression therapy and 20 healthy blood donors. The results showed that the frequency of IFN-γ +874 TT genotype in patients with aplastic anemia was significantly higher than the corresponding frequency in the healthy adults (42.6% vs. 17.6%, χ(2)=13.78, p=0. 01). The response rate in severe aplastic anemia patients with increased IFN-γ levels in the blood plasma was higher than that in severe aplastic anemia patients with decreased IFN-γ levels in the blood plasma (73.7% vs. 25.0%, p0.05). Of the 35 patients with severe aplastic anemia, 15 showed the IFN-γ +874 TT genotype, whereas response in 11 patients, the high response rate was significantly in the favor of the IFN-γ +874 TT genotype (73.3% vs. IFN-γ +874 non-TT 35%, p0.05). In conclusion, the IFN-γ +874T/A gene polymorphism may be correlated with response to immunosuppressive therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect