The rice CYP78A gene BSR2 confers resistance to Rhizoctonia solani and affects seed size and growth in Arabidopsis and rice
| Autor: | Satoru Maeda, Joseph G. Dubouzet, Hirohiko Hirochika, Masaki Mori, Kenji Oda, Shigemi Seo, Youichi Kondou, Minami Matsui, Yusuke Jikumaru |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Arabidopsis Gene Expression lcsh:Medicine Plant disease resistance Article Rhizoctonia Rhizoctonia solani 03 medical and health sciences 0302 clinical medicine Cytochrome P-450 Enzyme System Botany Gene expression Pseudomonas syringae lcsh:Science Gene Pathogen Colletotrichum higginsianum Disease Resistance Plant Diseases Multidisciplinary biology lcsh:R fungi food and beverages Oryza bacterial infections and mycoses equipment and supplies biology.organism_classification Recombinant Proteins 030104 developmental biology lcsh:Q 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) Scientific Reports SC30201902210008 NARO成果DBa OA CC BY |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-37365-1 |
| Popis: | The fungal pathogen Rhizoctonia solani causes devastating diseases in hundreds of plant species. Among these, R. solani causes sheath blight, one of the three major diseases in rice. To date, few genes have been reported that confer resistance to R. solani. Here, rice-FOX Arabidopsis lines identified as having resistance to a bacterial pathogen, Pseudomonas syringae pv. tomato DC3000, and a fungal pathogen, Colletotrichum higginsianum were screened for disease resistance to R. solani. BROAD-SPECTRUM RESISTANCE2 (BSR2), a gene encoding an uncharacterized cytochrome P450 protein belonging to the CYP78A family, conferred resistance to R. solani in Arabidopsis. When overexpressed in rice, BSR2 also conferred resistance to two R. solani anastomosis groups. Both Arabidopsis and rice plants overexpressing BSR2 had slower growth and produced longer seeds than wild-type control plants. In contrast, BSR2-knockdown rice plants were more susceptible to R. solani and displayed faster growth and shorter seeds in comparison with the control. These results indicate that BSR2 is associated with disease resistance, growth rate and seed size in rice and suggest that its function is evolutionarily conserved in both monocot rice and dicot Arabidopsis. |
| Databáze: | OpenAIRE |
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