Overexpression of phzM contributes to much more production of pyocyanin converted from phenazine-1-carboxylic acid in the absence of RpoS in Pseudomonas aeruginosa
| Autor: | Kewen Wang, Yanjie Yu, Xinyu Xu, Le Kai, Xiaoyan Chi, Kailu Zhang, Zhifen Yu, Yihe Ge, Mengyue Hao, Lijuan Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Virulence Factors
Phenazine Mutant Sigma Factor medicine.disease_cause Biochemistry Microbiology Virulence factor Mixed Function Oxygenases 03 medical and health sciences chemistry.chemical_compound Pyocyanin Bacterial Proteins Genetics medicine Humans Overproduction Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences 030306 microbiology Pseudomonas aeruginosa General Medicine Methyltransferases Enzyme chemistry Pyocyanine Phenazines rpoS |
| Zdroj: | Archives of microbiology. 202(6) |
| ISSN: | 1432-072X |
| Popis: | Pyocyanin produced by Pseudomonas aeruginosa is a key virulence factor that often causes heavy damages to airway and lung in patients. Conversion of phenazine-1-carboxylic acid to pyocyanin involves an extrametabolic pathway that contains two enzymes encoded, respectively, by phzM and phzS. In this study, with construction of the rpoS-deficient mutant, we first found that although phenazine production increased, pyocyanin produced in the mutant YTΔrpoS was fourfold much higher than that in the wild-type strain YT. To investigate this issue, we constructed phzM-lacZ fusion on a vector and on the chromosome. By quantifying β-galactosidase activities, we confirmed that expression of the phzM was up-regulated when the rpoS gene was inactivated. However, no changes occurred in the expression of phzS and phzH when the rpoS was knocked out. Taken together, overproduction of the SAM-dependent methyltransferase (PhzM) might contribute to the increased pyocyanin in the absence of RpoS in P. aeruginosa. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink