Genome Mining of the Lipopeptide Biosynthesis of Paenibacillus polymyxa E681 in Combination with Mass Spectrometry: Discovery of the Lipoheptapeptide Paenilipoheptin
| Autor: | Rainer Borriss, Joerg Doellinger, Max Weydmann, Peter Lasch, Joachim Vater, Stefanie Herfort |
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| Rok vydání: | 2018 |
| Předmět: |
Peptide Biosynthesis
0301 basic medicine medicine.drug_class Polymyxin 030106 microbiology Computational biology Biology Biochemistry Lipopeptides 03 medical and health sciences Paenibacillus chemistry.chemical_compound Bacterial Proteins Nonribosomal peptide Depsipeptides medicine Data Mining Polymyxins Molecular Biology Gene chemistry.chemical_classification Whole genome sequencing Organic Chemistry Computational Biology Lipopeptide biology.organism_classification chemistry Multigene Family Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Molecular Medicine Paenibacillus polymyxa Time-of-flight mass spectrometry |
| Zdroj: | ChemBioChem. 19:744-753 |
| ISSN: | 1439-4227 |
| DOI: | 10.1002/cbic.201700615 |
| Popis: | Paenibacillus polymyxa strains are qualified for agro-biotechnological uses such as plant growth promotion and for biocontrol strategies against deleterious phytopathogenic competitors in the soil depending on their attractive arsenal of bioactive compounds. Moreover, they are potent producers of antibiotics for medical applications. To identify new products of such organisms, genome mining strategies in combination with mass spectrometry are the methods of choice. Herein, we performed such studies with the Paenibacillus strain E681. Bioinformatic evaluation of its genome sequence revealed four gene clusters A-D encoding nonribosomal peptide synthetases (NRPSs). Accordingly, four lipopeptide families were detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Clusters A and D codify the well known fusaricidins and polymyxins. A yet-unknown lipoheptapeptide was discovered and structurally characterized by de novo sequencing by using MALDI-LIFT-TOF/TOF MS. It was designated as paenilipoheptin. From structure predictions we infer that the production of this agent is encoded by gene cluster C. Gene cluster B encodes the synthesis of tridecaptins, a family of open-chain lipotridecapeptides. Strain E681 produces new subspecies of such compounds (tridecaptins E) showing variations both in their fatty-acid part as well as in their peptide part. |
| Databáze: | OpenAIRE |
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