Characterization of cisplatin adducts of oligonucleotides by fast atom bombardment mass spectrometry
| Autor: | LeRoy B. Martin, Anton F. Schreiner, Richard B. van Breemen |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Binding Sites
Stereochemistry Oligonucleotide Oligonucleotides Biophysics chemistry.chemical_element Cell Biology Spectrometry Mass Fast Atom Bombardment Fast atom bombardment Mass spectrometry Biochemistry Adduct Gel permeation chromatography chemistry Tetramer Ionization Chromatography Gel Cisplatin Platinum Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | Analytical Biochemistry. 193:6-15 |
| ISSN: | 0003-2697 |
| Popis: | The products of the reaction of the antitumor drug cisplatin (cis-diamminedichloroplatinum(II)) with four oligonucleotide tetramers, d(GpCpGpC), d(GpGpCpC), d(TpGpApT), and d(TpGpCpT), were separated by gel permeation chromatography and characterized by negative- and positive-ion fast atom bombardment (FAB) mass spectrometry. Fragment ions indicating the oligonucleotide sequence and the position of cisplatin binding were observed in MS/MS spectra following collisional activation and B/E-linked scanning. Positive-ion FAB MS/MS spectra were characterized by platinum-containing product ions. Nonplatinated sequence ions and internal fragment ions were present primarily in the negative-ion spectra. The most prominent fragment ions containing platinum were [HB2·Pt·B3H]+ and [HB1·Pt·B2H]+, where B1, B2, and B3 were bases in the oligonucleotide tetramer, one of which was usually guanine. Both singly and doubly charged platinum complexes were observed, probably indicating reduction of Pt(II) during the FAB ionization process. The location of the platinum complex bound to each oligonucleotide sequence could be determined, and the binding sites observed by mass spectrometry were similar to those previously determined by other methods. FAB ionization with collisional activation and MS/MS analysis could serve as a new method for structural analysis of platinated oligonucleotides. |
| Databáze: | OpenAIRE |
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