Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion
| Autor: | Robert J. Porte, Basak E. Uygun, Korkut Uygun, James H. Avruch, Bote G. Bruinsma, Pepijn D. Weeder, Negin Karimian, Heidi Yeh, James F. Markmann, Gautham V. Sridharan |
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| Přispěvatelé: | Groningen Institute for Organ Transplantation (GIOT) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
ORGAN PRESERVATION
medicine.medical_specialty subnormothermic General Chemical Engineering Urology Portal vein Cold storage Economic shortage DONOR LIVERS 030230 surgery General Biochemistry Genetics and Molecular Biology Objective assessment 03 medical and health sciences 0302 clinical medicine Hepatic Artery Medicine Humans CRITERIA Issue 98 Machine perfusion General Immunology and Microbiology Human liver CHALLENGES business.industry Portal Vein TRANSPLANTATION General Neuroscience viability machine perfusion Tissue Donors Surgery Liver Transplantation Transplantation Cold Temperature Perfusion Liver CARDIAC DEATH 030211 gastroenterology & hepatology DONATION business Living donor liver transplantation |
| Zdroj: | Journal of Visualized Experiments. JOURNAL OF VISUALIZED EXPERIMENTS ISSUE=98;ISSN=1940-087X;TITLE=Journal of Visualized Experiments Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| Popis: | There is currently a severe shortage of liver grafts available for transplantation. Novel organ preservation techniques are needed to expand the pool of donor livers. Machine perfusion of donor liver grafts is an alternative to traditional cold storage of livers and holds much promise as a modality to expand the donor organ pool. We have recently described the potential benefit of subnormothermic machine perfusion of human livers. Machine perfused livers showed improving function and restoration of tissue ATP levels. Additionally, machine perfusion of liver grafts at subnormothermic temperatures allows for objective assessment of the functionality and suitability of a liver for transplantation. In these ways a great many livers that were previously discarded due to their suboptimal quality can be rescued via the restorative effects of machine perfusion and utilized for transplantation. Here we describe this technique of subnormothermic machine perfusion in detail. Human liver grafts allocated for research are perfused via the hepatic artery and portal vein with an acellular oxygenated perfusate at 21 degrees C. |
| Databáze: | OpenAIRE |
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