Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum

Autor: I.F. Kappers, Harro J. Bouwmeester, Benyamin Houshyani, Antoni Busquets, Albert Ferrer, Maryam Assareh
Přispěvatelé: Universitat de Barcelona, Research Foundation - Flanders, Ministerio de Ciencia e Innovación (España), European Commission
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Mecanismes de defensa en les plantes
Wasps
Genetically modified crops
lesion formation
Applied Microbiology and Biotechnology
farnesyl-diphosphate synthase
chemistry.chemical_compound
Linalool
Insectes paràsits
Arabidopsis thaliana
Laboratorium voor Plantenfysiologie
Laboratory of Entomology
terpenoid metabolism
Plant defenses
Terpens
food and beverages
Plants
Genetically Modified
PE&RC
Lepidoptera
defense
Enginyeria genètica vegetal
Metabolic Engineering
Biochemistry
Sesquiterpenes
Laboratory of Plant Physiology
Plant genetic engineering
Biotechnology
Transgene
Bioengineering
arabidopsis-thaliana
Biology
plant volatiles
Sesquiterpene
Host-Parasite Interactions
Biosynthesis
Arabis
Multienzyme Complexes
Botany
Animals
Nerolidol
Terpenes
fungi
mass-spectrometry
Àrabis
biology.organism_classification
Laboratorium voor Entomologie
herbivores
Terpenoid
Genetic Enhancement
chemistry
nicotiana-attenuata
biosynthesis
Parasitic insects
Zdroj: Recercat. Dipósit de la Recerca de Catalunya
instname
Digital.CSIC. Repositorio Institucional del CSIC
Metabolic Engineering, 15, 88-97
Metabolic Engineering 15 (2013)
Dipòsit Digital de la UB
Universidad de Barcelona
ISSN: 1096-7176
Popis: The concentration and ratio of terpenoids in the headspace volatile blend of plants have a fundamental role in the communication of plants and insects. The sesquiterpene (E)-nerolidol is one of the important volatiles with effect on beneficial carnivores for biologic pest management in the field. To optimize de novo biosynthesis and reliable and uniform emission of (E)-nerolidol, we engineered different steps of the (E)-nerolidol biosynthesis pathway in Arabidopsis thaliana. Introduction of a mitochondrial nerolidol synthase gene mediates de novo emission of (E)-nerolidol and linalool. Co-expression of the mitochondrial FPS1 and cytosolic HMGR1 increased the number of emitting transgenic plants (incidence rate) and the emission rate of both volatiles. No association between the emission rate of transgenic volatiles and their growth inhibitory effect could be established. (E)-Nerolidol was to a large extent metabolized to non-volatile conjugates.
This work was funded by the Earth and Life Sciences Council of the Netherlands Organization for Scientific Research (NWO-ALW) under the ERGO program (number 838.06.010) and a Technology Foundation grant (NWO-STW number 5479) to IFK. AF acknowledges funding by the Spanish Ministerio de Ciencia e Innovación BIO2009-06984 (including European Regional Development Funds) to and the Spanish Consolider-Ingenio 2010 Program (CSD2007-00036 Centre for Research in Agricultural Genomics).
Databáze: OpenAIRE
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