Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells
| Autor: | Ling-Hu Meng, Yue Liu, Jian-Quan Yang, Heng Wang, Zhen-Gang Tang, Yi Zhou, Cai-Li Li |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Angiogenesis OncoTargets and Therapy Umbilical vein quercetin angiogenesis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation metastasis heterocyclic compounds Pharmacology (medical) Original Research Tube formation Chemistry Cell growth glioblastoma Cell migration invasion Vascular endothelial growth factor A 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer research Quercetin |
| Zdroj: | OncoTargets and therapy |
| ISSN: | 1178-6930 |
| DOI: | 10.2147/ott.s136821 |
| Popis: | Yue Liu,1 Zhen-Gang Tang,1 Jian-Quan Yang,1 Yi Zhou,1 Ling-Hu Meng,1 Heng Wang,1 Cai-Li Li2 1Department of Neurosurgery, 2Institute of Esophageal Tumor, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, People’s Republic of China Abstract: Glioblastoma is the most aggressive type of brain tumor with a very poor prognosis. Therefore, it is always of great importance to explore and develop new potential treatment for glioblastoma. Quercetin, a flavonoid present in a variety of human foods, has been shown to inhibit various tumor cell proliferation. In this study, we found that treating human glioblastoma U251 cells with 10µg/mL quercetin for 24hours, a concentration that was far below the IC50 (113.65µg/mL) and at which quercetin failed to inhibit cell proliferation, inhibited cell migration (30%) and cell invasion as examined by wound scratch assay and transwell assay, respectively. We further showed that 10µg/mL quercetin inhibited cell migration and tube formation of human umbilical vein endothelial cells induced by the conditioned medium derived from U251 cell culture. The inhibitory effect of quercetin on migration and angiogenesis is possibly mediated through the downregulation of protein levels of VEGFA, MMP9, and MMP2 as detected by Western blot. Our findings demonstrated that low concentration of quercetin antagonized glioblastoma cell invasion and angiogenesis in vitro. Keywords: glioblastoma, quercetin, angiogenesis, invasion, metastasis |
| Databáze: | OpenAIRE |
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