Global Proximity Interactome of the Human Macroautophagy Pathway
| Autor: | Taoyingnan Li, Peter K. Kim, John H. Brumell, Julie D. Forman-Kay, Estelle M.N. Laurent, Rong Hua, Neale D. Ridgway, Andrew M. Sydor, Robert M. Vernon, Etienne Coyaud, Diana N Dyer, Nora Mellouk, Jonathan St-Germain, Brian Raught, Kexin Zhao, Yi Xin Iris Tu |
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| Přispěvatelé: | The Hospital for sick children [Toronto] (SickKids), Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 (PRISM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Dalhousie University [Halifax], University Health Network, Department of Molecular Genetics [Toronto], University of Toronto, INSERM, Université de Lille, Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192, The Hospital for sick children [Toronto] [SickKids] |
| Rok vydání: | 2021 |
| Předmět: |
membrane contact site
[SDV]Life Sciences [q-bio] oxysterol-binding protein-like Biology Interactome 03 medical and health sciences 0302 clinical medicine Salmonella Organelle Macroautophagy Autophagy Humans OSBPL11 BioID Molecular Biology OSBPL 030304 developmental biology 0303 health sciences Cell Biology Compartment (chemistry) Membrane contact site Cell biology OSBPL7 lipid-transfer proteins Plant lipid transfer proteins 030217 neurology & neurosurgery |
| Zdroj: | Autophagy Autophagy, 2021, Autophagy, 18 (5), pp.1-13. ⟨10.1080/15548627.2021.1965711⟩ |
| ISSN: | 1554-8635 1554-8627 |
| DOI: | 10.1080/15548627.2021.1965711⟩ |
| Popis: | International audience; Macroautophagy is a highly conserved eukaryotic cellular pathway involving the engulfment of macromolecules, organelles, and invading microbes by a double-membrane compartment and subsequent lysosomal degradation. The mechanisms that regulate macroautophagy, and the interaction of its components with other cellular pathways, have remained unclear. Here, we performed proximity-dependent biotin identification (BioID) on 39 core human macroautophagy proteins, identifying over 700 unique high confidence proximity interactors with new putative connections between macroautophagic and essential cellular processes. Of note, we identify members of the OSBPL (oxysterol binding protein like) family as Atg8-family protein interactors. We subsequently conducted comprehensive screens of the OSBPL family for LC3B-binding and roles in xenophagy and aggrephagy. OSBPL7 and OSBPL11 emerged as novel lipid transfer proteins required for macroautophagy of selective cargo. Altogether, our proximity interaction map provides a valuable resource for the study of autophagy and highlights the critical role of membrane contact site proteins in the pathway. |
| Databáze: | OpenAIRE |
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