Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification
| Autor: | Eric C. Liao, Lucie Rochard, Irving T.C. Ling |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Time Factors Meckel's cartilage Cartilage morphogenesis Biology Models Biological Chondrocyte Article 03 medical and health sciences Wnt Chondrocytes Osteogenesis medicine Animals Endochondral ossification Molecular Biology Wnt Signaling Pathway Zebrafish Cell Proliferation Ossification Osteoblast Muscles Skull Wnt signaling pathway Cell Polarity Gene Expression Regulation Developmental Cell Differentiation Anatomy Cell Biology Zebrafish Proteins Cell biology Wnt Proteins 030104 developmental biology medicine.anatomical_structure Cartilage Face Intramembranous ossification Mutation Joints medicine.symptom Chondrogenesis Developmental Biology |
| ISSN: | 0012-1606 |
| Popis: | Formation of the mandible requires progressive morphologic change, proliferation, differentiation and organization of chondrocytes preceding osteogenesis. The Wnt signaling pathway is involved in regulating bone development and maintenance. Chondrocytes that are fated to become bone require Wnt to polarize and orientate appropriately to initiate the endochondral ossification program. Although the canonical Wnt signaling has been well studied in the context of bone development, the effects of non-canonical Wnt signaling in regulating the timing of cartilage maturation and subsequent bone formation in shaping ventral craniofacial structure is not fully understood.. Here we examined the role of the non-canonical Wnt signaling pathway (wls, gpc4, wnt5b and wnt9a) in regulating zebrafish Meckel's cartilage maturation to the onset of osteogenic differentiation. We found that disruption of wls resulted in a significant loss of craniofacial bone, whereas lack of gpc4, wnt5b and wnt9a resulted in severely delayed endochondral ossification. This study demonstrates the importance of the non-canonical Wnt pathway in regulating coordinated ventral cartilage morphogenesis and ossification. |
| Databáze: | OpenAIRE |
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