Scutellarein from Scutellaria barbata induces apoptosis of human colon cancer HCT116 cells through the ROS-mediated mitochondria-dependent pathway
| Autor: | Fei Guo, Feng Yang, Yan-Hong Zhu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Membrane potential
biology 010405 organic chemistry Scutellarein Cytochrome c Organic Chemistry Plant Science Mitochondrion biology.organism_classification 01 natural sciences Biochemistry digestive system diseases 0104 chemical sciences Analytical Chemistry Cell biology Human colon cancer 010404 medicinal & biomolecular chemistry chemistry.chemical_compound chemistry Apoptosis biology.protein neoplasms Scutellaria barbata Intracellular |
| Zdroj: | Natural Product Research. 33:2372-2375 |
| ISSN: | 1478-6427 1478-6419 |
| DOI: | 10.1080/14786419.2018.1440230 |
| Popis: | To search novel therapy for human colon cancer, scutellarein identified from Scutellaria barbata was investigated using HCT116 cells. As a result, scutellarein can induce apoptosis of HCT116 cells. Further investigation for the mechanism has revealed scutellarein can increase the production of intracellular ROS and lead to the collapse of mitochondrial membrane potential. Meanwhile, the activity of caspase-3 in HCT116 cells was elevated by scutellarein. Moreover, down-regulated Bcl-2 and up-regulated Bax were observed. Additionaly, scutellarein resulted in cytochrome c release from mitochondria. These results indicated the apoptosis induction of HCT116 cells by scutellarein was implemented through ROS-mediated mitochondria-dependent pathway. |
| Databáze: | OpenAIRE |
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