Antibacterial activity of disodium succinoyl glycyrrhetinate, a derivative of glycyrrhetinic acid againstStreptococcus mutans
| Autor: | Takahito Yamashita, Shouichi Miyawaki, Atsuko Watanabe, Tamaki Katsumata, Yuichi Oogai, Yoshihiro Nishitani, Hitoshi Komatsuzawa, Miki Kawada-Matsuo |
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| Rok vydání: | 2019 |
| Předmět: |
Virulence Factors
Immunology Virulence Microbial Sensitivity Tests Biology Microbiology Streptococcus mutans 03 medical and health sciences Virology Glycyrrhiza Viability assay 030304 developmental biology 0303 health sciences Microbial Viability Plant Extracts 030306 microbiology Biofilm Gene Expression Regulation Bacterial PEP group translocation Hydrogen-Ion Concentration Carbohydrate biology.organism_classification Anti-Bacterial Agents Glucose Genes Bacterial Biofilms Glycyrrhetinic Acid Antibacterial activity Bacteria |
| Zdroj: | Microbiology and Immunology. 63:251-260 |
| ISSN: | 1348-0421 0385-5600 |
| DOI: | 10.1111/1348-0421.12717 |
| Popis: | Streptococcus mutans is a cariogenic bacterium that localizes in the oral cavity. Glycyrrhetinic acid (GRA) is a major component of licorice extract. GRA and several derivatives, including disodium succinoyl glycyrrhetinate (GR-SU), are known to have anti-inflammatory effects in humans. In this study, the antimicrobial effect of GRA and its derivatives against the S. mutans UA159 strain were investigated. Minimum inhibitory concentrations (MICs) of GRA and GR-SU showed antibacterial activity against the S. mutans strain, whereas other tested derivatives did not. Because GR-SU is more soluble than GRA, GR-SU was used for further experiments. The antibacterial activity of GR-SU against 100 S. mutans strains was evaluated and it was found that all strains are susceptible to GR-SU, with MIC values below 256 µg/mL. A cell viability assay showed that GR-SU has a bacteriostatic effect on S. mutans cells. As to growth kinetics, sub-MICs of GR-SU inhibited growth. The effect of GR-SU on S. mutans virulence was then investigated. GR-SU at sub-MICs suppresses biofilm formation. Additionally, GR-SU greatly suppresses the pH drop caused by the addition of glucose and glucose-induced expression of the genes responsible for acid production (ldh and pykF) and tolerance (aguD and atpD). Additionally, expression of enolase, which is responsible for the carbohydrate phosphotransferase system, was not increased in the presence of GR-SU, indicating that GR-SU suppresses incorporation of sugars into S. mutans. In conclusion, GR-SU has antibacterial activity against S. mutans and also decreases S. mutans virulence. |
| Databáze: | OpenAIRE |
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