Evaluation of ELISA-Based Multiplex Peptides for the Detection of Human Serum Antibodies Induced by Zika Virus Infection across Various Countries
| Autor: | Laise de Moraes, Aruna D. De Silva, Tamara García-Salum, Kennedy T. L. Gifford, Kimberly García, Daniel G. Olson, Viviane Boaventura, Lillian Nunes Gomes, Daniela Weiskopf, Gene S. Tan, Guillermo M. Ruiz-Palacios, Stephen Panossian, Kevan Akrami, Antônio Augusto Moura da Silva, Maria del Pilar Martinez Viedma, Ivette Lorenzana, Rafael A. Medina, Brett E. Pickett, Isis Figueroa, Cecilia Perret, Ricardo Khouri, Alessandro Sette, Leda Parham |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent 030231 tropical medicine Enzyme-Linked Immunosorbent Assay Cross Reactions Antibodies Viral Sensitivity and Specificity Microbiology Neutralization Article Zika virus Serology Cohort Studies 03 medical and health sciences Young Adult 0302 clinical medicine antibody diagnostic Humans Multiplex zika virus Child Aged Aedes biology Zika Virus Infection seropositivity Outbreak Infant bioinformatics Middle Aged biology.organism_classification Virology QR1-502 High-Throughput Screening Assays virology Flavivirus 030104 developmental biology Infectious Diseases Immunoglobulin M Child Preschool Immunoglobulin G biology.protein Female ELISA Antibody Peptides |
| Zdroj: | Viruses Volume 13 Issue 7 Viruses, Vol 13, Iss 1319, p 1319 (2021) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v13071319 |
| Popis: | Zika virus (ZIKV) is a mosquito-borne Flavivirus with a positive-sense RNA genome, which are generally transmitted through the bite of an infected Aedes mosquito. ZIKV infections could be associated with neurological sequelae that, and otherwise produces similar clinical symptoms as other co-circulating pathogens. Past infection with one member of the Flavivirus genus often induces cross-reactive antibodies against other flaviruses. These attributes complicate the ability to differentially diagnose ZIKV infection from other endemic mosquito-borne viruses, making it both a public health issue as well as a diagnostic challenge. We report the results from serological analyses using arbovirus-specific peptides on 339 samples that were previously collected from 6 countries. Overall, we found that our multiplexed peptide-based ELISA was highly efficient for identifying ZIKV antibodies as early as 2 weeks post infection, and that it correlates with microneutralization, plaque reduction neutralization tests (PRNTs) and commercial tests for ZIKV in previously characterized samples. We observed that seropositivity varied by patient cohort, reflecting the sampling period in relation to the 2015–2016 ZIKV outbreak. This work evaluates the accuracy, specificity, and sensitivity of our peptide-based ELISA method for detecting ZIKV antibodies from geographically diverse regions. These findings can contribute to ongoing serological methods development and can be adapted for use in future studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |