Targeted delivery of functionalized PLGA nanoparticles to macrophages by complexation with the yeast Saccharomyces cerevisiae
| Autor: | Marcus Koch, Marc Schneider, Manfred J. Schmitt, Frank Breinig, Ruth Kiefer, Charlotte Dahlem, Alexandra K. Kiemer, Marijas Jurisic |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cell type Carrier system Cell Survival Saccharomyces cerevisiae Bioengineering yeast 01 natural sciences Applied Microbiology and Biotechnology HeLa 03 medical and health sciences chemistry.chemical_compound Immune system Drug Delivery Systems Phagocytosis Polylactic Acid-Polyglycolic Acid Copolymer 010608 biotechnology Humans biology Macrophages technology industry and agriculture biology.organism_classification nanomedicine Yeast Coculture Techniques Cell biology PLGA 030104 developmental biology chemistry drug delivery Drug delivery Nanoparticles Immunotherapy Biotechnology HeLa Cells |
| Zdroj: | Biotechnology and bioengineeringREFERENCES. 117(3) |
| ISSN: | 1097-0290 |
| Popis: | Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering the body. Therefore, we investigated the interaction of biodegradable surface-modified poly(lactic-co-glycolic acid) (PLGA) particles with yeast cells to overcome the unspecificity of the particulate carriers. Cells of different Saccharomyces cerevisiae strains were characterized regarding their interaction with PLGA-NPs under isotonic and hypotonic conditions. The particles were shown to efficiently interact with yeast cells leading to stable NP/yeast-complexes allowing to associate or even internalize compounds. Notably, applying those complexes to a coculture model of HeLa cells and macrophages, the macrophages were specifically targeted. This novel nano-in-micro carrier system suggests itself as a promising tool for the delivery of biologically active agents into phagocytic cells combining specificity and efficiency. |
| Databáze: | OpenAIRE |
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