Transcriptional profiling of liver tissues in chicken embryo at day 16 and 20 using RNA sequencing reveals differential antioxidant enzyme activity
| Autor: | Xiaoqian Ou, Lu Lu Wang, Wei Wang, Guoqing Liu, Xue Chen, Shaohua Yang, Zhaoyuan Shi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Embryology Transcription Genetic GPX3 Molecular biology lcsh:Medicine Gene Expression Chick Embryo Biochemistry Antioxidants Transcriptome Oxidative Damage Sequencing techniques 0302 clinical medicine Gene expression Medicine and Health Sciences lcsh:Science Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Gene Ontologies Heart RNA sequencing Embryo Genomics Glutathione Liver 030220 oncology & carcinogenesis embryonic structures Anatomy Research Article animal structures Biology Real-Time Polymerase Chain Reaction GSTA4 Superoxide dismutase 03 medical and health sciences Genetics Animals Glutathione Peroxidase Sequence Analysis RNA Superoxide Dismutase Gene Expression Profiling lcsh:R Embryos Biology and Life Sciences Computational Biology Genome Analysis Enzyme assay Research and analysis methods Gene expression profiling Molecular biology techniques 030104 developmental biology Cardiovascular Anatomy biology.protein lcsh:Q Peptides Reactive Oxygen Species Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 2, p e0192253 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Considering the high proportion of polyunsaturated fatty acids, the antioxidant defense of chick embryo tissues is vital during the oxidative stress experienced at hatching. In order to better understand the mechanisms of the defense system during chicken embryo development, we detected the activity of antioxidant enzymes during the incubation of chicken embryo. Results showed that the activity of superoxide dismutase (SOD) and (GSH-PX) in livers were higher than those in hearts. Based on these results, liver tissues were used as the follow-up study materials, which were obtained from chicken embryo at day 16 and day 20. Thus, we used RNA sequencing (RNA-Seq) analysis to identify the transcriptome from 6 liver tissues. In total, we obtained 45,552,777-45,462,856 uniquely mapped reads and 18,837 mRNA transcripts, across the 6 liver samples. Among these, 1,154 differentially expressed genes (p |
| Databáze: | OpenAIRE |
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