A Withanolide-rich Fraction of Athenaea velutina Induces Apoptosis and Cell Cycle Arrest in Melanoma B16F10 Cells
Autor: | Leandro Licursi de Oliveira, Juliana Alves do Vale, Ana C. Zanatta, Gustavo Costa Bressan, Alisson Andrade Almeida, Marinês Eiterer, João Paulo Viana Leite, Laís Azevedo Rodrigues, Graziela Domingues de Almeida Lima |
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Přispěvatelé: | Universidade Federal de Viçosa (UFV), Independent Researcher, Universidade Estadual Paulista (Unesp) |
Rok vydání: | 2021 |
Předmět: |
Athenaea velutina
Cell cycle checkpoint Pharmaceutical Science Apoptosis 01 natural sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Tandem Mass Spectrometry Cell Line Tumor geographic distribution Drug Discovery Humans Cytotoxic T cell Propidium iodide Fluorescein isothiocyanate Melanoma Withanolides Solanaceae Cell Proliferation 030304 developmental biology Pharmacology 0303 health sciences Plant Extracts 010405 organic chemistry Cell growth Cell Cycle Organic Chemistry apoptosis Cell Cycle Checkpoints Cell cycle Molecular biology 0104 chemical sciences Complementary and alternative medicine chemistry cell cycle arrest withanolides Cancer cell Molecular Medicine |
Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
ISSN: | 1439-0221 0032-0943 |
Popis: | Made available in DSpace on 2021-06-25T10:28:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Athenaea velutina is a promising Brazilian shrub with cytotoxic and antimigratory properties against cancer cells. However, the mechanism of induction of cancer cell death and the compounds involved remain unknown. To ascertain these bioactive compounds, bioassay-guided fractionation was performed, alongside the appropriate in vitro tests. A withanolide-rich fraction (F Av 5) from the dichloromethane extract increased cytotoxic activity by 1.5-fold (IC 50= 2.1 μg/mL). Fourteen withanolide steroids were tentatively identified for the first time for this species by mass spectrometry coupled to liquid chromatography (LC MS/MS), including withanolide A, aurelianolide A, and aurelianolide B. F Av 5 significantly decreased cell proliferation, migration, and invasion with a selectivity index greater than 8 for B16F10 cells. Furthermore, flow cytometry with annexin V fluorescein isothiocyanate/propidium iodide (V-FITC/PI) staining showed F Av 5 to promote cell cycle arrest at the G0/G1-phase as well as apoptotic cell death. Overall, these findings highlight A. velutina as a source of withanolide-steroids that inhibit cancer cell proliferation through apoptosis and cell cycle blockade mechanisms. Details on the geographic distribution of A. velutina and species conservation strategies have also been highlighted. Departamento de Bioquímica e Biologia Molecular Universidade Federal de Viçosa Departamento de Biologia Geral Universidade Federal de Viçosa Independent Researcher Universidade Estadual Paulista (Unesp) Instituto de Química Universidade Estadual Paulista (Unesp) Instituto de Química |
Databáze: | OpenAIRE |
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