Trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: potential use in polyhydroxyalkanoate production
| Autor: | Gustavo J. Vazquez, M. J. Pettinari, N. Krüger, Alexander Steinbüchel, Patricia S. Vary, Beatriz S. Méndez |
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| Rok vydání: | 1998 |
| Předmět: |
Transcriptional Activation
Operon Polyesters Molecular Sequence Data Biology medicine.disease_cause Applied Microbiology and Biotechnology Open Reading Frames medicine Escherichia coli Amino Acid Sequence Regulator gene Bacillus megaterium Sequence Homology Amino Acid Activator (genetics) fungi Structural gene General Medicine Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Complementation Open reading frame Biochemistry Genes Bacterial bacteria Sequence Alignment Biotechnology Plasmids |
| Zdroj: | Applied microbiology and biotechnology. 49(6) |
| ISSN: | 0175-7598 |
| Popis: | A Bacillus megaterium genomic fragment, which encoded an activator homologous to sigma 54 regulators and which was capable of activating Escherichia coli ato genes in trans, was detected in a gene library of B. megaterium screened for beta-ketothiolase activity. The fragment presented only one complete open reading frame (ORF1), which encoded a protein of 398 amino acids. The recombinant plasmid complemented mutations in the Escherichia coli atoC regulatory gene. The constitutive expression of the E. coli ato operon mediated by ORF1 could be useful for the synthesis of polyhydroxyalkanoates with different flexibility properties by recombinant E. coli strains. |
| Databáze: | OpenAIRE |
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