Evaluation of CD4+CD25+FoxP3+ T cell populations, IL-10 production, and their correlation with clinical and biochemical parameters in sickle cell anemia patients with leg ulcers
| Autor: | Aderson S Araujo, Maira Galdino da Rocha Pitta, Marcos André Cavalcanti Bezerra, Moacyr Jesus Barreto de Melo Rêgo, Michelly Cristiny Pereira, Ivan da Rocha Pitta, Diego Arruda Falcão, Rafael Ramos da Silva |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male T cell Immunology Population Anemia Sickle Cell T-Lymphocytes Regulatory Biochemistry Peripheral blood mononuclear cell Young Adult Th2 Cells Immunophenotyping medicine Humans Immunology and Allergy IL-2 receptor education Molecular Biology Cells Cultured Inflammation education.field_of_study business.industry Leg Ulcer Interleukin-2 Receptor alpha Subunit Osteonecrosis FOXP3 Forkhead Transcription Factors hemic and immune systems Hematology Middle Aged Th1 Cells medicine.disease Sickle cell anemia Interleukin-10 Interleukin 10 medicine.anatomical_structure Leukocytes Mononuclear Female business |
| Zdroj: | Cytokine. 75:310-315 |
| ISSN: | 1043-4666 |
| Popis: | Leg ulcers (LUs) are a debilitating complication of sickle cell anemia (SCA), with inflammation known to play a crucial role in their pathogenesis. Many studies have described the roles of T helper type 1 (Th1) and Th2 pathways in SCA; however, defects in anti-inflammatory responses are poorly understood. We evaluated interleukin (IL)-10 levels in serum and peripheral blood mononuclear cells (PBMCs) in SCA patients with leg ulcers (SCALU) and without leg ulcers (SCAWH) in addition to CD4(+) CD25(+)FoxP3(+) T cell populations and their its IL-10 expression. In stimulated and unstimulated PBMC cultures, SCALU patients produced higher levels of IL-10 than those in the SCAWH group. Higher levels of IL-10 in SCALU patients correlated with a history of osteonecrosis in stimulated and unstimulated cultures when compared with those in SCAWH. Immunophenotyping revealed that SCALU patients had a higher proportion of CD4(+)CD25(+)FoxP3(+), Tr1 and CD4(+)CD25(+)FoxP3(+)IL-10(+) T cells than other groups. Our findings revealed that IL-10 levels were increased in unstimulated cells from the SCALU group, and that this group also presented with a predominant CD4(+) CD25(+)FoxP3(+) cell population despite many of those cells being IL-10 negative. |
| Databáze: | OpenAIRE |
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