Antigen recognition properties of a Vγ1.3Vδ2-T-cell receptor from a rare variant of polymyositis
| Autor: | Georg Dechant, Reinhard Hohlfeld, Klaus Dornmair, Heinz Wiendl, Joachim Malotka, Christian Schneider |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
T cell
Immunology Epitopes T-Lymphocyte Autoimmunity chemical and pharmacologic phenomena Biology Major histocompatibility complex Epitope Antigen Escherichia coli medicine Animals Humans Immunology and Allergy Receptor Cells Cultured Antigens Bacterial T-cell receptor Receptors Antigen T-Cell gamma-delta hemic and immune systems Surface Plasmon Resonance Molecular biology Polymyositis medicine.anatomical_structure Neurology biology.protein Neurology (clinical) Bacterial antigen Antibody Protein Binding |
| Zdroj: | Journal of Neuroimmunology. 152:168-175 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/j.jneuroim.2004.03.016 |
| Popis: | Previously we partially characterized an autoreactive human Vgamma1.3Vdelta2-T-cell receptor (TCR) that had originally been identified in muscle of a patient with an unusual form of polymyositis. This TCR recognizes a muscle-associated auto-antigen in a CDR3-dependent, MHC non-restricted way. Here we show that this TCR also recognizes an antigen from Escherichia coli. Like the muscle-associated mammalian antigen, the bacterial antigen is recognized in a CDR3-dependent, but MHC-non-restricted way. Both antigens have strikingly similar molecular characteristics suggesting that their epitopes are at least very similar. The dissociation kinetics of the bacterial antigen-TCR complexes was investigated by surface plasmon resonance using soluble single-chain TCR molecules produced in COS-7 cells. The measured dissociation rate constant (k(off)=5.7 x 10(-3) s(-1)) shows that the complexes dissociate more slowly than most previously described antigen/alphabeta-TCR complexes, but much faster than antibody/antigen pairs. These results (a) provide further insight into the molecular properties of this unusual TCR, and (b) should help in future attempts to identify the elusive target antigen(s). |
| Databáze: | OpenAIRE |
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