Effect of Growth Factor Supplementation on the Hair Cell Specific Markers of Cells Harvested from Basilar Membrane
| Autor: | Kien Hui Chua, Muhd Azhan Ubaidah, Aminuddin Bin Saim, Ruszymah Binti Haji Idrus, Azida Zainal, Mazita Ami, Lokman Saim, Yogeswaran Lokanathan |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_treatment
Cell morphology Mice SOX2 Epidermal growth factor Hair Cells Auditory otorhinolaryngologic diseases medicine Animals Progenitor cell Cells Cultured business.industry Growth factor Cell Differentiation General Medicine Nestin Immunohistochemistry Basilar Membrane Cell biology Culture Media medicine.anatomical_structure Otorhinolaryngology Intercellular Signaling Peptides and Proteins sense organs Hair cell Stem cell business |
| Zdroj: | The journal of international advanced otology. 11(1) |
| ISSN: | 1308-7649 |
| Popis: | OBJECTIVE Loss of auditory hair cells is a major cause of deafness. The presence of auditory progenitor cells in the inner ear raises the hope for mammalian inner ear cell regeneration. In this study, we aimed to investigate the effect of growth factor supplementations, namely a combination of epidermal growth factor (EGF), insulin-like growth factor (IGF), and beta (β)-fibroblast growth factor (βFGF), on the expression of hair cell-specific markers by cells harvested from the cochlear membrane. This would provide an insight into the capability of these cells to differentiate into hair cells. MATERIALS AND METHODS EGF, IGF, and βFGF were supplemented into the culture medium. The cells were evaluated by morphology, growth kinetic, gene expression, and protein expression. RESULTS The cultured cells of mouse basilar membrane were spindle shaped. Growth factors-enriched medium promotes a significantly higher proliferative activity than the basic culture medium but did not alter the cell morphology. Growth factors-enriched medium did not show any significant differences in the protein expression of the hair cell-specific markers myosin VIIa and calretinin and the stem-cell marker nestin. Gene expression analysis showed that the expression of the hair cell-specific genes myosin VIIa and calretinin as well as the stem cell genes nestin, Rex1, and Sox2 was reduced after the cells were passaged in the growth factor-supplemented medium. Cells in the basic medium expressed a significantly higher level of hair cell-specific genes at certain passages. CONCLUSION Growth factor supplementation could not maintain the expression of hair cell-specific markers by cells obtained from the cochlear membrane. |
| Databáze: | OpenAIRE |
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