A critical appraisal of humanized alternatives to fetal bovine serum for clinical applications of umbilical cord derived mesenchymal stromal cells
| Autor: | Rajasekaran Subbarayan, Suneel Rallapalli, Santosh Mathapati, Purna Sai Korrapati, Soma Guhathakurta, Dillip Kumar Bishi |
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| Rok vydání: | 2021 |
| Předmět: |
Population
Cell Culture Techniques Bioengineering Applied Microbiology and Biotechnology Umbilical cord Flow cytometry Umbilical Cord Andrology Osteogenesis medicine Animals Humans CD90 education Cells Cultured Cell Proliferation education.field_of_study biology medicine.diagnostic_test CD44 Mesenchymal stem cell Mesenchymal Stem Cells Serum Albumin Bovine General Medicine Fetal Blood Culture Media medicine.anatomical_structure biology.protein Cattle Fetal bovine serum Ex vivo Biotechnology |
| Zdroj: | Biotechnology letters. 43(10) |
| ISSN: | 1573-6776 |
| Popis: | The study is aimed to verify the possibility of using humanized alternatives to fetal bovine serum (FBS) such as umbilical cord blood plasma (CBP) and AB+ plasma to support the long-term growth of mesenchymal stromal cells (MSCs) derived from the umbilical cord. We hypothesized that umbilical CBP would be a potential substitute to FBS, especially for small scale autologous clinical transplantations. The MSCs were cultured for six consecutive passages to evaluate xeno-free media's ability to support long-term growth. Cell proliferation rates, colony-forming-unit (CFU) efficiency and population doublings of expanded MSCs, were investigated. Ex vivo expanded MSCs were further characterized using flow cytometry and quantitative PCR. The impact of cryopreservation and composition of cryomedium on phenotype, viability of MSC was also assessed. Our results on cell proliferation, colony-forming unit efficiency suggested that the expansion of the cells was successfully carried out in media supplemented with humanized alternatives. MSCs showed lower CFU counts in FBS (~ 25) than humanized alternatives (~ 35). The gene expression analysis revealed that transcripts showed significant differential expression by two to three folds in the FBS group compared with MSCs grown in medium with humanized alternatives (p |
| Databáze: | OpenAIRE |
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