Identification of an S-antigen-like molecule in Drosophila melanogaster: An immunohistochemical study
| Autor: | Wolfgang E. Lieb, Harminder S Dua, Alan C. Christensen, Larry A. Donoso, Laura Smith-Lang |
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| Rok vydání: | 1991 |
| Předmět: |
genetic structures
Blotting Western Mutant Biology Autoantigens Photoreceptor cell Cellular and Molecular Neuroscience biology.animal Drosophilidae medicine Animals Amino Acid Sequence Antigens Drosophila (subgenus) Eye Proteins Arrestin Vertebrate Compound eye biology.organism_classification Immunohistochemistry Molecular biology eye diseases Sensory Systems Cell biology Ophthalmology Drosophila melanogaster medicine.anatomical_structure Visual phototransduction |
| Zdroj: | Experimental Eye Research. 53:171-178 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/0014-4835(91)90071-l |
| Popis: | Vision is a highly complex process common to many vertebrate and invertebrate species. The visual system of Drosophila represents a valuable model in which to study this process since its chromosomal makeup has been well mapped and many mutants containing specific photoreceptor defects have been described. S-antigen is a well-characterized photoreceptor cell protein which plays a fundamental role in the visual process. In this study, we used a panel of four monoclonal antibodies (MAbs) specific for amino, mid and carboxy terminal amino acid sequences in S-antigen in order to examine S-antigen immunoreactivity in the Drosophila visual system. Two MAbs, MAb 5C6.47 and MAbC10-C10, localized S-antigen to the retinules and ocelli. In contrast, MAbA2-G5 and MAbA9-C6 did not stain. The presence and strong antigenic similarity of an S-antigen homolog in Drosophila suggests that in both vertebrates and invertebrates S-antigen plays a major role in the regulation of the phototransduction of vision. |
| Databáze: | OpenAIRE |
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