Sonogenetics is a non-invasive approach to activating neurons in Caenorhabditis elegans
Autor: | Sreekanth H. Chalasani, Sadik C. Esener, Carolyn E. Schutt, Stuart Ibsen, Ada Tong |
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Rok vydání: | 2015 |
Předmět: |
Sensory Receptor Cells
General Physics and Astronomy Sensory system Optogenetics Stimulus (physiology) General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Interneurons Biological neural network Animals Mechanotransduction Caenorhabditis elegans Caenorhabditis elegans Proteins TRPC Cation Channels 030304 developmental biology Neurons 0303 health sciences Microbubbles Multidisciplinary biology General Chemistry Anatomy biology.organism_classification 3. Good health Ultrasonic Waves nervous system Neuroscience Locomotion 030217 neurology & neurosurgery |
Zdroj: | Nature Communications. 6 |
ISSN: | 2041-1723 |
Popis: | A major challenge in neuroscience is to reliably activate individual neurons, particularly those in deeper brain regions. Current optogenetic approaches require invasive surgical procedures to deliver light of specific wavelengths to target cells to activate or silence them. Here, we demonstrate the use of low-pressure ultrasound as a non-invasive trigger to activate specific ultrasonically sensitized neurons in the nematode, Caenorhabditis elegans. We first show that wild-type animals are insensitive to low-pressure ultrasound and require gas-filled microbubbles to transduce the ultrasound wave. We find that neuron-specific misexpression of TRP-4, the pore-forming subunit of a mechanotransduction channel, sensitizes neurons to ultrasound stimulus, resulting in behavioural outputs. Furthermore, we use this approach to manipulate the function of sensory neurons and interneurons and identify a role for PVD sensory neurons in modifying locomotory behaviours. We suggest that this method can be broadly applied to manipulate cellular functions in vivo. |
Databáze: | OpenAIRE |
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