Effects of High Glucose on the Expression of LAMA1 and Biological Behavior of Choroid Retinal Endothelial Cells
| Autor: | Haihua Zheng, Licheng Bao, Qianying Gao, Da Lin, Guangwei Song, Qi Jiang, Yinan Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Article Subject Endocrinology Diabetes and Metabolism Alpha (ethology) 030209 endocrinology & metabolism Immunofluorescence lcsh:Diseases of the endocrine glands. Clinical endocrinology Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Cell Movement Laminin Cell Adhesion medicine Animals Cell Proliferation Basement membrane lcsh:RC648-665 Dose-Response Relationship Drug medicine.diagnostic_test biology Choroid Cell growth Chemistry Endothelial Cells Retinal Diabetic retinopathy medicine.disease Macaca mulatta Cell biology Blot Glucose 030104 developmental biology medicine.anatomical_structure biology.protein Research Article |
| Zdroj: | Journal of Diabetes Research Journal of Diabetes Research, Vol 2018 (2018) |
| ISSN: | 2314-6753 2314-6745 |
| DOI: | 10.1155/2018/7504614 |
| Popis: | Hyperglycemia is one of the main causes of proliferative diabetic retinopathy (PDR) characterized by thickening of the vascular basement membrane. Laminin alpha 1 (LAMA1) is a primary component of laminin, a major protein constituent of the basement membrane. In this study, we investigated the role of LAMA1 in the development of PDR. Retinal choroidal vascular endothelial cells (RF/6A line) were exposed to glucose at different concentrations (5 mM, 15 mM, 25 mM, and 35 mM) and analyzed for cell growth, migration, proliferation, and adhesion. LAMA1 expression was examined 24 and 48 h following glucose treatment using Western blotting, RT-PCR, and immunofluorescence. The results showed that the proliferation, migration, and adhesion of RF/6A cells were increased by high glucose, whereas LAMA1 expression was slightly higher at 15 mM but decreased at 25 mM and 35 mM glucose compared to control. Thus, the changes in the biological behavior of high glucose-exposed retinal vascular endothelial cells correspond to variations in LAMA1 expression, indicating a possibility for LAMA1 involvement in PDR development. Our findings suggest that LAMA1 may play a role in PDR and, thus, may serve as a potential target for DR diagnosis and/or treatment. |
| Databáze: | OpenAIRE |
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