Comparative Analysis of Electrochemiluminescence Assay and Chemiluminescent Microparticle Immunoassay for the Screening of Hepatitis C
| Autor: | Maeesa Wadood, Muhammad Usman |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Population 030204 cardiovascular system & hematology Gastroenterology Asymptomatic law.invention 03 medical and health sciences 0302 clinical medicine law Positive predicative value Internal medicine medicine education Chemiluminescence education.field_of_study Hematology medicine.diagnostic_test biology business.industry Hepatitis C medicine.disease Immunoassay biology.protein Original Article Antibody medicine.symptom business 030215 immunology |
| Zdroj: | Indian Journal of Hematology and Blood Transfusion. 35:131-136 |
| ISSN: | 0974-0449 0971-4502 |
| DOI: | 10.1007/s12288-018-0968-3 |
| Popis: | The primary objective of the study was to identify a highly sensitive and specific screening technique for the detection of Hepatitis C infection in healthy blood donors in a low prevalence area for HCV. In this study, two of the most commonly used methods for Anti-HCV screening, i.e., Electrochemiluminescence Immunoassay (ECLIA) and Chemiluminescent Microparticle Immunoassay (CMIA) were performed among 517 selected healthy blood donors. The clinical performance of ECLIA and CMIA was compared on the basis of their operational variables, i.e., Sensitivity, Specificity, Accuracy, Youden’s J index, Positive and Negative predictive values and False discovery, False positive and False negative rate, etc., Both ECLIA and CMIA are highly sensitive (100%) and specific (98%) in terms of anti HCV detection among the blood donors. According to the clinical performance of ECLIA and CMIA, both are efficient in detecting anti-HCV antibodies among the asymptomatic population of healthy blood donors. But as both of them are associated with false positive results, it is recommended to have Polymerase chain reaction on the reactive samples to detect the HCV RNA. |
| Databáze: | OpenAIRE |
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