Modeling of C-SNARF-1 pH fluorescence properties: towards calibration free optical fiber pH sensing for in vivo applications
| Autor: | Samlee Mankhetkorn, Bernard Gauthier-Manuel, Bruno Wacogne, Christian Pieralli, Rutjaphan Kateklum |
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| Přispěvatelé: | Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph]
Optical fiber Chemistry law In vivo Ph sensing Analytical chemistry [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics Fluorescence Calibration free law.invention [SPI.MAT]Engineering Sciences [physics]/Materials |
| Zdroj: | International Conference on Biomedical Electronics and Devices International Conference on Biomedical Electronics and Devices, Jan 2018, Funchal, Portugal BIODEVICES |
| Popis: | International audience; Organic functions of the human body are related to biological constants. Variations of these constants, among them pH, induce pathological troubles. The general goal of our work is to fabricate a fluorescent pH sensor at the end of an optical fiber for in vivo pH measurements. One difficulty using fluorescence indicators is the need to perform an accurate calibration. In this communication, we present methods used to simplify and potentially avoid calibration procedures of fluorescence indicators. The first method concerns the simplification of calibration procedures making them independent of the indicator’s concentration, path length and equipment used. The second method concerns modelling the fluorescence emission of the molecules as a function of pH only. This model is used to fit the exact shape of C-SNARF-1 fluorescence spectra obtained at any pH. Subsequently, the pH of a solution can be computed with an accuracy of 0.1 pH unit without the calibration procedure employed up to now. These methods constitute the first steps toward calibration free pH measurements. They can be applied to any fluorescent indicator exhibiting a dual emission peak. As a conclusion, this is the first time that fluorescence properties of C-SNARF-1 are fully mathematically described. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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