Isolation and characterization of polymorphic microsatellite markers in Bagarius yarrelli using RNA-Seq
| Autor: | Du M, Niu Bz, Liu Yh |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Genetics
education.field_of_study Polymorphism Genetic biology Sequence Analysis RNA Population Locus (genetics) General Medicine biology.organism_classification Animals Microsatellite Polymorphic Microsatellite Marker Nucleotide Motifs Allele Bagarius yarrelli education Molecular Biology Allele frequency Alleles Catfishes Microsatellite Repeats Catfish |
| Zdroj: | Genetics and Molecular Research. 14:16308-16311 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/2015.december.8.21 |
| Popis: | The yellow sisorid catfish (Bagarius yarrelli) is a vulnerable fish species. In this study, seven polymorphic microsatellite DNA markers for yellow sisorid catfish were described, using RNA-Seq methodology. In B. yarrelli (N = 44) from a Hekou wild population, allelic frequency, and observed and expected heterozygosities per locus varied from two to six, 0.0333 to 0.6793, and 0.0333 to 0.6004, respectively. One locus (Baya153) denoted notable separation from the Hardy-Weinberg equilibrium, after sequential Bonferroni correction (P < 0.05). The microsatellite markers described here will be useful for investigating population structure and genetic resource of B. yarrelli from different geographical locations. |
| Databáze: | OpenAIRE |
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