Relationship between thiol, tyrosine nitration and carbonyl formation as biomarkers of oxidative stress and changes of hemostatic function of plasma from breast cancer patients before surgery
| Autor: | Janusz Piekarski, Barbara Wachowicz, Magdalena Kedzierska, Beata Olas, Arkadiusz Jeziorski |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
medicine.medical_specialty Nitrosation Protein Carbonylation Clinical Biochemistry Breast Neoplasms medicine.disease_cause Antioxidants Fibrin Breast cancer Biomarkers Tumor medicine Humans Neoplasm Invasiveness Platelet Sulfhydryl Compounds skin and connective tissue diseases Hemostatic function Aged Hemostasis biology Chemistry General Medicine Middle Aged medicine.disease Blood proteins Surgery Oxidative Stress Case-Control Studies biology.protein Tyrosine Female Oxidative stress |
| Zdroj: | Clinical Biochemistry. 45:231-236 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2011.12.002 |
| Popis: | Objectives In breast cancer patients dysregulation of reactive oxygen species metabolism, as detected by various indicators in plasma or various blood cells, including red blood cells and blood platelets, is observed. Design and methods The aim of our study was to explain the effect of oxidative/nitrative stress on hemostatic activity of plasma from breast cancer patients. Different methods were used to analyze oxidative/nitrative stress (carbonyl groups and 3-nitrotyrosine levels - ELISA and C-ELISA method, respectively and free thiol groups was estimated with 5,5′-dithio-bis(2-nitro-benzoic acid)). We also measured total antioxidant status (TAS) in plasma. Results Our results showed the correlation between the oxidative stress and changes of hemostasis in breast cancer patients; the correlation between the increased amount of carbonylated or nitrated plasma proteins and fibrin polymerization or lysis in plasma isolated from invasive breast cancer patients. We also observed that the relationship between TAS and fibrin polymerization or lysis in plasma from invasive breast cancer exists. Conclusion Considering the data presented in this study, we suggest that the oxidative/nitrative stress in breast cancer patients may induce the changes of hemostasis in these patients. |
| Databáze: | OpenAIRE |
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