Genotoxic and Cytotoxic Effects in Exfoliated Buccal and Nasal Cells of Chromium and Cobalt Exposed Electroplaters
| Autor: | Michael Kundi, Miroslav Mišík, Pavel Vodicka, Georg Wultsch, Armen Nersesyan, Monika Waldherr, Tahereh Setayesh, Ludmila Vodickova, Siegfried Knasmüller |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Chromium Male 0301 basic medicine Karyolysis DNA damage Health Toxicology and Mutagenesis Mucous membrane of nose Toxicology 03 medical and health sciences Occupational Exposure Chromosomes Human Humans Cytotoxic T cell Micronuclei Chromosome-Defective Cell Nucleus Cell Death Chemistry Karyorrhexis Mouth Mucosa Cobalt Electroplating Molecular biology Nasal Mucosa 030104 developmental biology Micronucleus test Female Chromium toxicity Pyknosis |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 80:651-660 |
| ISSN: | 1087-2620 1528-7394 |
| Popis: | Results of a number of studies indicate that electroplaters have increased cancer risks as a consequence of exposure to genotoxic metals such as chromium (VI) and nickel. These effects may be due to induction of damage of the genetic material which plays a key role in the etiology of cancer, and it was found that workers in galvanization factories exhibited increased levels of DNA damage. The aim of the present study was to investigate genetic stability in workers of a bright plating factory who are exposed to chromium (Cr) and cobalt (Co). Exfoliated cells were collected from the buccal and nasal mucosa of workers (n = 42) and matched controls (n = 43) and analyzed for induction of micronuclei (MN) which are formed as a consequence of chromosomal aberrations. In addition, other nuclear anomalies namely nuclear buds (Nbuds) which are formed as a consequence of gene amplification and markers indicating different stages of cell death (condensed chromatin, karyorrhexis, karyolysis, and pyknosis) were also assessed. No evidence was noted for induction of MN, but significantly increased rates of Nbuds in cells from both, buccal and nasal mucosa, were found. Parameters which are indicative for cytotoxic effects were more pronounced in nasal cells and rose with duration of employment period. Overall, our findings indicated that no apparent chromosomal damage occurred in bright electroplaters. However, data demonstrated that acute cytotoxic effects may lead to inflammations and/or lesions in epithelia of the respiratory tract of the workers. |
| Databáze: | OpenAIRE |
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