Database independent detection of isotopically labeled MS/MS spectrum peptide pairs
| Autor: | Frank Potthast, Martin Pelikan, Dorothea Rutishauser, Jiri Ocenasek, Ralph Schlapbach |
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| Rok vydání: | 2005 |
| Předmět: |
chemistry.chemical_classification
Chromatography Database Molecular Sequence Data Clinical Biochemistry Peptide Cell Biology General Medicine Open source software computer.software_genre Mass spectrometry Proteomics Biochemistry Mass Spectrometry Analytical Chemistry chemistry Complex protein Peptide mass fingerprinting De novo sequencing Amino Acid Sequence Peptides computer Peptide sequence Algorithms |
| Zdroj: | Journal of Chromatography B. 817:225-230 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2004.12.009 |
| Popis: | Mass spectrometry data generated in differential profiling of complex protein samples are classically exploited using database searches. In addition, quantitative profiling is performed by various methods, one of them using isotopically coded affinity tags, where one typically uses a light and a heavy tag. Here, we present a new algorithm, ICATcher, which detects pairs of light/heavy peptide MS/MS spectra independent of sequence databases. The method can be used for de novo sequencing and detection of posttranslational modifications. ICATcher is distributed as open source software. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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