Effect of hepato-toxins in the acceleration of hepatic fibrosis in hepatitis B mice

Autor: Doo Hyun Kim, Myeong Ju Moon, Ra-Young Park, Reju George Thomas, Suchithra Poilil Surendran, Yong Yeon Jeong, Kyun-Hwan Kim
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Physiology
Thioacetamide
Pharmacology
Liver Cirrhosis
Experimental
medicine.disease_cause
Biochemistry
Mice
chemistry.chemical_compound
0302 clinical medicine
Fibrosis
Medicine and Health Sciences
Pathology and laboratory medicine
Mice
Inbred C3H
Multidisciplinary
Liver Diseases
Hepatotoxin
Hep G2 Cells
Animal Models
Transfection
Medical microbiology
Hepatitis B
Body Fluids
Blood
Liver
Experimental Organism Systems
030220 oncology & carcinogenesis
Viruses
Liver Fibrosis
Medicine
Female
Anatomy
Pathogens
Plasmids
Research Article
Hepatitis B virus
Science
Mouse Models
Gastroenterology and Hepatology
Research and Analysis Methods
Biomolecular isolation
Microbiology
03 medical and health sciences
Model Organisms
medicine
Animals
Humans
Molecular Biology Techniques
Molecular Biology
Ethanol
business.industry
Viral pathogens
Organisms
Biology and Life Sciences
Proteins
medicine.disease
DNA isolation
Hepatic stellate cell activation
Hepatitis viruses
digestive system diseases
Microbial pathogens
Mice
Inbred C57BL
030104 developmental biology
chemistry
Animal Studies
business
Hepatic fibrosis
Collagens
Developmental Biology
Zdroj: PLoS ONE, Vol 15, Iss 5, p e0232619 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Chronic liver diseases such as hepatitis B viral (HBV) infection and liver fibrosis have been a major health problem worldwide. However, less research has been conducted owing to the lack of animal models. The key purpose of this study was to determine the effects of different hepatotoxins in HBV-affected liver. In this study, we successfully generated a combined liver fibrosis model by administering HBV 1.2 plasmid and thioacetamide/ethanol (TAA/EtOH). To our knowledge, this is the first study in which an increase in the liver fibrosis level is observed by the intraperitoneal administration of TAA and EtOH in drinking water after the hydrodynamic transfection of the HBV 1.2 plasmid in C3H/HeN mice. The HBV+TAA/EtOH group exhibited higher level of hepatic fibrosis than that of the control groups. The hepatic stellate cell activation in the TAA- and EtOH-administered groups was demonstrated by the elevation in the level of fibrotic markers. In addition, high levels of collagen content and histopathological results were also used to confirm the prominent fibrotic levels. We established a novel HBV mice model by hydrodynamic injection-based HBV transfection in C3H/HeN mice. C3H/HeN mice were reported to have a higher HBV persistence level than that of the C57BL/6 mouse model. All the results showed an increased fibrosis level in the HBV mice treated with TAA and EtOH; hence, this model would be useful to understand the effect of hepatotoxins on the high risk of fibrosis after HBV infection. The acceleration of liver fibrosis can occur with prolonged administration as well as the high dosage of hepatotoxins in mice.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje