Pseudomonas aeruginosa gbdR gene is transcribed from a σ54-dependent promoter under the control of NtrC/CbrB, IHF and BetI
| Autor: | Diego Germán Sánchez, Emiliano David Primo, María Teresa Damiani, Angela T. Lisa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Integration Host Factors GBDR Transcription Genetic Otras Ciencias Biológicas 030106 microbiology Regulator Biology Microbiology Ciencias Biológicas 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Transcription (biology) Transcriptional regulation Choline Binding site P. AERUGINOSA Promoter Regions Genetic Gene Transcription factor RPON TRANSCRIPTIONAL REGULATION Gene Expression Regulation Bacterial BETI chemistry Biochemistry NTRC Mutation Pseudomonas aeruginosa rpoN CHOLINE CIENCIAS NATURALES Y EXACTAS Protein Binding |
| Zdroj: | Microbiology (Reading, England). 163(9) |
| ISSN: | 1465-2080 |
| Popis: | Pseudomonasaeruginosa uses choline as a source of carbon and nitrogen, and also for the synthesis of glycine betaine, an osmoprotectant under stress conditions such as drought and salinity. The transcription factor GbdR is the specific regulator of choline metabolism and it belongs to the Arac/XylS family of transcriptional regulators. Despite the link between choline catabolism and bacterial pathogenicity, gbdR regulation has not been explored in detail. In the present work, we describe how gbdR transcription can be initiated from a s 54-dependent promoter. gbdR transcription can be activated by NtrC in the absence of a preferential nitrogen source, by CbrB in the absence of a preferential carbon source, and by the integration host factor favouring DNA bending. In addition, we found that BetI negatively regulates gbdR expression in the absence of choline. We identified two overlapping BetI binding sites in the gbdR promoter sequence, providing an additional example of s 54-promoter down-regulation. Based on our findings, we propose a model for gdbR regulation and its impact on choline metabolism. Fil: Sanchez, Diego German. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Primo, Emiliano David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Lisa, Angela Teresita. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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