Increased IL-21/IL-21R expression and its proinflammatory effects in autoimmune thyroid disease
| Autor: | Jin-an Zhang, Xiaohong Shi, Shuai Meng, Ling Xiao, Liangfeng Shi, Jian Xu, Li-juan Guan, Xuan Wang, Wen-juan Jiang |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty CD3 Immunology Thyroid Gland Hashimoto Disease Real-Time Polymerase Chain Reaction Biochemistry Peripheral blood mononuclear cell Thyroiditis Proinflammatory cytokine Pathogenesis Interferon-gamma Immune system Internal medicine medicine Immunology and Allergy Humans Molecular Biology biology business.industry Interleukins Thyroid Interleukin-17 Hematology Middle Aged medicine.disease Flow Cytometry Immunohistochemistry Graves Disease Recombinant Proteins Endocrinology medicine.anatomical_structure biology.protein Leukocytes Mononuclear Receptors Interleukin-21 Interleukin-4 business CD8 |
| Zdroj: | Cytokine. 72(2) |
| ISSN: | 1096-0023 |
| Popis: | To determine the potential role of interleukin-21 (IL-21) / IL-21 receptor (IL-21R) in the pathogenesis of autoimmune thyroid disease (AITD) mainly known as Graves' disease (GD) and Hashimoto's thyroiditis (HT). IL-21 and IL-21R of peripheral blood samples and/or thyroid tissues from AITD patients and healthy controls were analyzed by ELISA, quantitative real-time polymerase chain reaction (qRT-PCR), flow cytometry and immunohistochemistry. In vitro, the mRNA and protein of inflammatory cytokines of cultured peripheral blood mononuclear cells (PBMCs) upon recombinant human IL-21 (rhIL-21) stimulation were detected. There was an increased serum concentration of IL-21 in untreated GD and HT patients, and IL-21(+)CD3(+)CD8(-)T cells were significantly increased in PBMCs of HT patients compared with healthy volunteers. The IL-21 mRNA expression in PBMCs increased dramatically in GD and HT patients, and marked augmentations of IL-21 and IL-21R mRNA in thyroid tissues of HT patients were observed. Immunohistochemical staining confirmed the expression of IL-21R protein in HT thyroid cells and lymphocytes. In vitro, PBMCs from GD cultured with rhIL-21 induced increased IL-17A but decreased IL-4 production, while from HT stimulated by rhIL-21 induced augmented production of IFN-γ. In conclusion, the expression of IL-21 and IL-21R were up-regulated in AITD and may be involved in the pathogenesis of the disease through augmenting aberrant immune cascade. |
| Databáze: | OpenAIRE |
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