Evaluation of poly([2‐(acryloyloxy)ethyl]trimethylammonium chloride) cationic polymer capillary coating for capillary electrophoresis and electrokinetic chromatography separations

Autor:	Julie R. McGettrick, Christopher P. Palmer
Rok vydání:	2017
Předmět:	chemistry.chemical_classification Hexadimethrine bromide Chromatography Chemistry 010401 analytical chemistry Cationic polymerization Filtration and Separation 02 engineering and technology Polymer 021001 nanoscience & nanotechnology 01 natural sciences Chloride 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Electrokinetic phenomena Capillary electrophoresis Polymerization medicine Zeta potential 0210 nano-technology medicine.drug
Zdroj:	Journal of Separation Science. 40:4060-4066
ISSN:	1615-9314 1615-9306
DOI:	10.1002/jssc.201700461
Popis:	Capillary electrophoresis and electrokinetic chromatography are typically carried out in unmodified fused-silica capillaries under conditions that result in a strong negative zeta potential at the capillary wall and a robust cathodic electroosmotic flow. Modification of the capillary wall to reverse the zeta potential and mask silanol sites can improve separation performance by reducing or eliminating analyte adsorption, and is essential when conducting electrokinetic chromatography separations with cationic latex nanoparticle pseudo-stationary phases. Semipermanent modification of the capillary walls by coating with cationic polymers has proven to be facile and effective. In this study, poly([2-(acryloyloxy)ethyl]trimethylammonium chloride) polymers were synthesized by reversible addition-fragmentation chain transfer polymerization and used as physically adsorbed semipermanent coatings for capillary electrophoresis and electrokinetic chromatography separations. An initial synthesis of poly([2-(acryloyloxy)ethyl]trimethylammonium chloride) polymer coating produced strong and stable anodic electroosmotic flow of -5.7 to -5.4 × 10-4 cm2 /V⋅s over the pH range of 4-7. Significant differences in the magnitude of the electroosmotic flow and effectiveness were observed between synthetic batches, however. For electrokinetic chromatography separations, the best performing batches of poly([2-(acryloyloxy)ethyl]trimethylammonium chloride) polymer performed as well as the commercially available cationic polymer polyethyleneimine, whereas polydiallylammonium chloride and hexadimethrine bromide did not perform well.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e0f333f7f2aac0c193fd527a636a697 https://doi.org/10.1002/jssc.201700461 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.