Chiral Os(II) Polypyridyl Complexes as Enantioselective Nuclear DNA Imaging Agents Especially Suitable for Correlative High-Resolution Light and Electron Microscopy Studies
| Autor: | Ben-Zhan Zhu, Tao Xu, Li Qin, Bo Shao, Yanhong Xue, Rong Huang, Fengping Feng, Miao Tang, Chun-Hua Huang, Li Mao, Zhu-Ying Yan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Osmium Tetroxide Mitosis 02 engineering and technology 010402 general chemistry 01 natural sciences law.invention chemistry.chemical_compound Microscopy Electron Transmission law Cell Line Tumor Animals Humans General Materials Science Cell Nucleus Microscopy Enantioselective synthesis Cationic polymerization Stereoisomerism DNA 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences Nuclear DNA chemistry Transmission electron microscopy Reagent Electron microscope 0210 nano-technology Phenanthrolines |
| Zdroj: | ACS applied materialsinterfaces. 12(3) |
| ISSN: | 1944-8252 |
| Popis: | The high-resolution technique transmission electron microscopy (TEM), with OsO4 as the traditional fixative, is an essential tool for cell biology and medicine. Although OsO4 has been extensively used, it is far from perfect because of its high volatility and toxicity. Os(II) polypyridyl complexes like [Os(phen)2(dppz)]2+ (phen = 1,10-phenanthroline; dppz = dipyridophenazine) are not only the well-known molecular DNA “light-switches” but also the potential ideal candidates for TEM studies. Here, we report that the cell-impermeable cationic [Os(phen)2(dppz)]2+ can be preferentially delivered into the live-cell nucleus through ion-pairing with chlorophenolate counter-anions, where it functions as an unparalleled enantioselective nuclear DNA imaging reagent especially suitable for correlative light and electron microscopy (CLEM) studies in both living and fixed cells, which can clearly visualize chromosome aggregation and decondensation during mitosis simultaneously. We propose that the chiral Os(II) polypyr... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|