In Vitro and In Silico Analysis of miR-125a with rs12976445 Polymorphism in Breast Cancer Patients
Autor: | Paweł P. Jagodziński, Joanna Miskiewicz, Sylwia Grodecka-Gazdecka, Natalia Szostak, Tomasz P. Lehmann, Marta Szachniuk |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty In silico lcsh:Technology polymorphism lcsh:Chemistry 03 medical and health sciences Prostate cancer 0302 clinical medicine Breast cancer breast cancer Internal medicine Genotype microRNA medicine General Materials Science RNA folding Instrumentation Gene lcsh:QH301-705.5 Pneumonitis Fluid Flow and Transfer Processes business.industry lcsh:T Process Chemistry and Technology General Engineering medicine.disease RNA protein binding In vitro lcsh:QC1-999 Computer Science Applications 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 030220 oncology & carcinogenesis business lcsh:Engineering (General). Civil engineering (General) lcsh:Physics |
Zdroj: | Applied Sciences, Vol 10, Iss 7275, p 7275 (2020) Applied Sciences Volume 10 Issue 20 |
ISSN: | 2076-3417 |
Popis: | Background: Breast cancer affects over 2 million women yearly. Its early detection allows for successful treatment, which motivates to research factors that enable an accurate diagnosis. miR-125a is one of them, correlating with different types of cancer. For example, the miR-125a level decreases in breast cancer tissues polymorphisms in the miR-125a encoding gene are related to prostate cancer and the risk of radiotherapy-induced pneumonitis. Methods: In this work, we investigated two variants of rs12976445 polymorphism in the context of breast cancer. We analyzed the data of 175 blood samples from breast cancer patients and compared them with the control data from 129 control samples. Results: We observed the tendency that in breast cancer cases TT genotype appeared slightly more frequent over CC and CT genotypes (statistically nonsignificant). The TT genotype appeared also to be more frequent among human epidermal growth factor receptor 2 (HER2) positive patients, compared to HER2 negative. In silico modelling showed that the presence of uridine (U) diminished the probability of pri-miR-125a binding to NOVA1 and HNRNPK proteins. We demonstrated that U and C-variants could promote different RNA folding patterns and provoke alternative protein binding. Conclusions: U-variant may imply a lower miR-125a expression in breast cancer. |
Databáze: | OpenAIRE |
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