Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. Manihotis strain CIO151
| Autor: | Tristan Boureau, Stéphane Poussier, Cesar Medina, Jens Boch, Prabhu B. Patil, Mariana Restrepo Benavides, Laurent D. Noël, Isabelle Robène-Soustrade, Sébastien Carrère, Lionel Gagnevin, Camilo López, Luis M. Rodriguez-R, Nathalia Arias Rojas, Armelle Darrasse, Ana C. Díaz, Cesar Trujillo, Juan F. Ortiz Quiñones, Robert P. Ryan, Nathaly Andrea Montenegro, Lucie Poulin, Perrine David, Fabien Guérin, Valérie Verdier, Boris Szurek, Olivier Pruvost, Mario L. Arrieta-Ortiz, Thomas Dugé de Bernonville, Daniela Osorio, Silvia Restrepo, Emmanuelle Lauber, Alejandra Munoz Bodnar, Carolina Pardo, Alvaro L. Pérez-Quintero, Pierre Lefeuvre, Oscar G. Urrego Morales, Adriana Bernal, Marie-Agnès Jacques, Paula Fontanilla, Christian Vernière, Rebecca Bart, Javier F. Tabima, Ralf Koebnik, Edgar M. Medina |
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| Přispěvatelé: | Universidad de Los Andes [Venezuela] (ULA), Institut de Recherche pour le Développement (IRD), Universidad Nacional de Colombia [Bogotà] (UNAL), Lawrence Berkeley National Laboratory [Berkeley] (LBNL), Martin-Luther-University Halle-Wittenberg, PRES Université Nantes Angers Le Mans (UNAM), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), UMR Peuplement Végétaux et Bioagresseurs en Milieu Tropical (UMR PVBMT - INRA), Institut National de la Recherche Agronomique (INRA), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de La Réunion (UR)-Institut National de la Recherche Agronomique (INRA), University of Dundee, Centre IRD de Montpellier, Colorado State University [Fort Collins] (CSU), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR), Colombian Administrative Department of Science and Technology (Colciencias) [264-2008], Vicechancellors office for research at Universidad de los Andes, International Foundation of Science [C/4074-1], Faculty of Sciences, INRA, department SPE, CIRAD, French Agence National de la Recherche [ANR-2010-BLAN-1723, ANR-2010-GENM-013, ANR-10-JCJC-1703-01, ANR-08-BLAN-0193-01], French Direction Generale de l'Armement [2011 60 091], Marie Curie IOF Fellowship (EU Grant) at Colorado State University (Bioagricultural Sciences and Plant Management) [PIOF-GA-2009-235457], NIFA (National Institute of Food and Agriculture) [2011-67012-30662], Universidad de Los Andes [Mérida, Venezuela] (ULA) |
| Rok vydání: | 2013 |
| Předmět: |
Xanthomonas axonopodis pv. manihotis (Xam)
Phylogénie Résistance génétique Manihot esculenta lcsh:Medicine Minisatellite Repeats Xanthomonas axonopodis pv. Manihotis Strain CIO151 Genome manioc F30 - Génétique et amélioration des plantes Xanthomonas citri Interactions biologiques infection pathogène Xam strain CIO151 Marqueur génétique lcsh:Science Pathotype Genetics 0303 health sciences Multidisciplinary biology Virulence Xanthomonas campestris facteur de pathogénicité PCR Xanthomonas euvesicatoria Pathovar Xanthomonas axonopodis Determinantes de patogenicidad bactérie pathogène Research Article Locus des caractères quantitatifs Xanthomonas Séquence nucléotidique Pouvoir pathogène Genomics approche génomique 03 medical and health sciences H20 - Maladies des plantes 030304 developmental biology Génie génétique Whole genome sequencing Génome 030306 microbiology lcsh:R Résistance aux maladies biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy Gène Marcadores VNTR lcsh:Q Genome Bacterial |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (11), pp.e79704. ⟨10.1371/journal.pone.0079704⟩ PLoS ONE, Vol 8, Iss 11, p e79704 (2013) PLoS ONE, 2013, 8 (11), pp.e79704. ⟨10.1371/journal.pone.0079704⟩ PLoS One Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario Plos One 11 (8), . (2013) PloS One |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0079704⟩ |
| Popis: | l pasado 21 de abril de 2009 la Honorable Consiliatura dio su aprobación al proyecto denominado Escuela de Medicina y Ciencias de la Salud Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of bacterial blight of cassava, which is among the main components of human diet in Africa and South America. Current information about the molecular pathogenicity factors involved in the infection process of this organism is limited. Previous studies in other bacteria in this genus suggest that advanced draft genome sequences are valuable resources for molecular studies on their interaction with plants and could provide valuable tools for diagnostics and detection. Here we have generated the first manually annotated high-quality draft genome sequence of Xam strain CIO151. Its genomic structure is similar to that of other xanthomonads, especially Xanthomonas euvesicatoria and Xanthomonas citri pv. citri species. Several putative pathogenicity factors were identified, including type III effectors, cell wall-degrading enzymes and clusters encoding protein secretion systems. Specific characteristics in this genome include changes in the xanthomonadin cluster that could explain the lack of typical yellow color in all strains of this pathovar and the presence of 50 regions in the genome with atypical nucleotide composition. The genome sequence was used to predict and evaluate 22 variable number of tandem repeat (VNTR) loci that were subsequently demonstrated as polymorphic in representative Xam strains. Our results demonstrate that Xanthomonas axonopodis pv. manihotis strain CIO151 possesses ten clusters of pathogenicity factors conserved within the genus Xanthomonas. We report 126 genes that are potentially unique to Xam, as well as potential horizontal transfer events in the history of the genome. The relation of these regions with virulence and pathogenicity could explain several aspects of the biology of this pathogen, including its ability to colonize both vascular and non-vascular tissues of cassava plants. A set of 16 robust, polymorphic VNTR loci will be useful to develop a multi-locus VNTR analysis scheme for epidemiological surveillance of this disease. |
| Databáze: | OpenAIRE |
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