Physostigmine modulation of acetylcholine currents in COS cells transfected with mouse muscle nicotinic receptor
| Autor: | Vyskocil F, Tomáš Hendrych, Jan Krůšek, Lucie Svobodová |
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| Rok vydání: | 2006 |
| Předmět: |
Agonist
medicine.medical_specialty Physostigmine Patch-Clamp Techniques medicine.drug_class Genetic Vectors Neuromuscular Junction Receptors Nicotinic Biology Transfection Synaptic Transmission Ion Channels Membrane Potentials Mice Desensitization (telecommunications) Internal medicine Chlorocebus aethiops medicine Animals Patch clamp Muscle Skeletal Receptor Acetylcholine receptor Dose-Response Relationship Drug General Neuroscience Cell Membrane Drug Synergism Acetylcholine Endocrinology Nicotinic agonist COS Cells Biophysics Cholinesterase Inhibitors medicine.drug |
| Zdroj: | Neuroscience Letters. 401:20-24 |
| ISSN: | 0304-3940 |
| Popis: | Physostigmine (Phy), a reversible inhibitor of acetylcholine (ACh) esterase (AChE), may also act as a low potency agonist and a modulator of the nicotinic receptor. The actions of Phy on mouse muscle nicotinic receptors in the COS-7 cell line were studied by the patch-clamp technique. Currents were recorded in the whole-cell mode 3-7 days after cell transfection by plasmids coding alphabetagammadelta combination of receptor subunits. The application of ACh to cells clamped at -10 mV produced inward currents which displayed desensitization. The application of Phy in concentrations up to 1 x 10(-3) M did not give reliable specific whole-cell membrane responses. The application of Phy in concentrations of 10(-6)-10(-4) M together with ACh modulated the amplitude; accelerated desensitization of currents induced by ACh and increased the final extent of desensitization in a concentration-dependent manner. This finding is in contrast to the suppression and slowing down of desensitization by Phy and 1-methyl-galanthamine observed in Torpedo receptors. |
| Databáze: | OpenAIRE |
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