Intracellular and extracellular protective mechanisms of the anammox consortia against exogenous sulfadimidine
Autor: | Yuan-Long, Ma, Zheng-Yang, Lu, Jin-Jin, Fu, Nian-Si, Fan, Ren-Cun, Jin |
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Rok vydání: | 2022 |
Předmět: |
Environmental Engineering
Nitrogen Health Toxicology and Mutagenesis Drug Resistance Microbial Sulfamethazine Wastewater Pollution Anaerobic Ammonia Oxidation Anti-Bacterial Agents Molecular Docking Simulation Bioreactors Animals Environmental Chemistry Oxidation-Reduction Waste Management and Disposal |
Zdroj: | Journal of Hazardous Materials. 434:128817 |
ISSN: | 0304-3894 |
Popis: | Antibiotics and antibiotic resistance genes (ARGs) have been recognized as emerging high-risk pollutants for human and animal health. This study systematically investigated the comprehensive effects of a typical antibiotic (sulfadimidine, SDM) in livestock and poultry breeding wastewater on the anammox process, with the aim of elucidating the intracellular and extracellular protective mechanisms of the anammox consortia to the antibiotic stress. Results revealed that the high-concentration SDM significantly reduced the specific anammox activity (SAA) by 37.8%. Changes in the abundance of Candidatus Kuenenia showed a similar trend with that of SAA, while other nitrogen-related microorganisms (e.g., Nitrosomonas and Nitrospira) contributed to the nitrogen removal especially during the inhibitory period. Resistance of the anammox consortia to SDM mainly depended on the protection of ARGs and EPS. Network analysis revealed the host range of eARGs was relatively larger than that of iARGs, and intI1 was closely associated with representative denitrifiers. In addition, metaproteomic analysis and molecular docking results indicated that abundant proteins in EPS could detain SDM in the extracellular matrix through forming complex via hydrogen bond. These findings provide a guidance for the stable operation of anammox process and ARGs transfer controlling. |
Databáze: | OpenAIRE |
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